LIM2551
12070505, human colon, epithelial; forms aggregates of small rounded cells, adherent and suspended.
Synonym(s):
LIM 2551, LIM-2551
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About This Item
UNSPSC Code:
41106514
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product name
LIM2551, 12070505
biological source
human colon
growth mode
Semi-adherent
karyotype
Not specified
morphology
Epithelial; grows as loose aggregates of small rounded cells, some adherent, others in suspension.
receptors
Not specified
relevant disease(s)
cancer
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Cell Line Origin
Human derived from an adenocarcinoma of the transverse colon
Cell Line Description
LIM2551 was derived from an adenocarcinoma of the transverse colon. Cell Bank Australia reference number: CBA0170.
DNA Profile
Not specified
Culture Medium
RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442), Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM.
Subculture Routine
Optimal split ratio 1:8-1:16 (seeding density 1 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. LIM2551 requires the growth medium to be changed 3 times each week. Feed and passage as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne, Australia
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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