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C0897

Sigma-Aldrich

Citrate Lyase from Klebsiella pneumoniae

lyophilized powder, ≥0.20 unit/mg solid

Synonym(s):

Citrate oxaloacetate lyase

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About This Item

CAS Number:
9012-83-3
Enzyme Commission number:
4.1.3.6 (BRENDA, IUBMB)
EC Number:
232-740-7
MDL number:
MFCD00130807
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

specific activity

≥0.20 unit/mg solid

mol wt

>5 kDa

storage temp.

2-8°C

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Application

Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).

Biochem/physiol Actions

Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).

Unit Definition

One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Djamel Drider et al.
Genetics and molecular research : GMR, 3(2), 273-281 (2004-07-22)
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert
M Hupperich et al.
European journal of biochemistry, 192(1), 161-166 (1990-08-28)
The primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from Klebsiella pneumoniae (ATCC 13,882) was determined with protein chemical methods. The polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 Da.
M Martín et al.
FEMS microbiology letters, 174(2), 231-238 (1999-05-26)
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF
Citric acid mediates the iron absorption from low molecular weight human milk fractions
Palika R, et al.
Journal of Agricultural and Food Chemistry, 61(46), 11151-11157 (2013)
Julia Gaye-Siessegger et al.
Isotopes in environmental and health studies, 40(3), 181-190 (2004-09-17)
The use of stable isotope techniques for the reconstruction of diets has increased over the last decade. However, isotopic ratios in an animal are not only affected by the composition of the feed, but also by the amount of feed
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