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Key Documents

137260

SAFC

BIS-TRIS

EMPROVE® EVOLVE

Pharma Manufacturing

Synonym(s):

BIS TRIS, 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

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About This Item

Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
Molecular Weight:
209.24
Beilstein:
2205275
MDL number:
UNSPSC Code:
12161705

biological source

synthetic

Quality Level

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals, tested

pH

5.8-7.2

useful pH range

5.8-7.2

pKa (25 °C)

6.5

pKa 

6.5

solubility

water: 0.1 M, clear, colorless (aqueous)

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

storage temp.

2-30°C

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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Application

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological based formulations.Bis Tris is used in a wide variety of biological applications including culture media, electrophoresis separations and diagnostic reagent formulations.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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