T7293

M-Gredient^® Casein Peptone for Biotechnology and Microbiology

Pancreatically Digested, for Biotechnology and Microbiology, 11.0-16.0% Total N

• Synonym(s): Peptone from casein, Peptone from casein, Pancreatic digest of casein., Tryptone
• CAS Number: 91079-40-2
• UNSPSC Code: 41106212
• NACRES: NA.74
• EC Number: 293-428-4

Properties

• Product Name: Tryptone, Pancreatically Digested
• biological source: bovine milk
• Quality Segment: 200
• form: powder
• quality: Pancreatically Digested
• packaging: bottle of 1 kg, bottle of 250 g
• nitrogen analysis: 11.0-16.0% total
• loss: ≤6% loss on drying
• color: light yellow
• pH: 6.9-7.4
• application(s): biotechnology/fermenter; microbiology
• suitability: Escherichia coli, Haemophilus spp., Lactobacillus spp., Streptococcus spp., coliforms

Description

General description

Tryptone is a mixture of peptides that is commonly used as a nutrient source in microbiological culture media. It is derived from soybean casein digest and contains a complex mixture of amino acids and peptides, including tryptophan. Tryptone is often used in combination with other ingredients, such as yeast extract and agar, to provide a nutrient-rich environment for the growth of microorganisms. It is particularly useful for culturing fastidious bacteria, which require a more complex nutrient source than simple sugars or amino acids. Tryptone is also used as a component of some enrichment broths and is a common ingredient in commercial media formulations.

Application

Tryptone has a wide range of applications in microbiology, including:

• Growth media: Tryptone is frequently used as a source of nutrients in growth media for bacteria, yeast, and other microorganisms. It can be used alone or in combination with other ingredients, such as agar, glucose, and salts, to provide a complete nutrient source for microbial growth
• Enrichment media: Tryptone is often added to enrichment broths to enhance the growth of slow-growing or stressed bacteria in clinical or environmental samples. This is particularly useful for samples where the target microbe is present in low numbers or is difficult to isolate
• Production of recombinant proteins: Tryptone is a component of many bacterial growth media used to produce recombinant proteins. It provides a rich source of amino acids and peptides, which are needed for protein biosynthesis
• It is also used in microbiological testing to determine the minimum inhibitory concentration (MIC) of antimicrobial agents against test organisms.Overall, the diverse and versatile properties of Tryptone make it a valuable tool in various microbiological applications

Preparation Note

Pancreatic digest of casein.

Legal Information

M-GREDIENT is a registered trademark of Merch KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)