P9375

1,10-Phenanthroline monohydrate

reagent grade

• Synonym(s): o-Phenanthroline monohydrate
• Empirical Formula (Hill Notation): C₁₂H₈N₂ · H₂O
• CAS Number: 5144-89-8
• Molecular Weight: 198.22
• Beilstein: 4008096
• EC Number: 200-629-2
• MDL number: MFCD00149973
• UNSPSC Code: 12352116
• PubChem Substance ID: 24278663
• NACRES: NA.77

Properties

• biological source: synthetic (organic)
• Quality Level: 200
• grade: reagent grade
• form: powder
• mp: 100-104 °C (lit.)
• solubility: methanol: 200 mM (Stock solution is stable for months at –20° C.)
• SMILES string: O.c1cnc2c(c1)ccc3cccnc23
• InChI: 1S/C12H8N2.H2O/c1-3-9-5-6-10-4-2-8-14-12(10)11(9)13-7-1;/h1-8H;1H2
• InChI key: PPQJCISYYXZCAE-UHFFFAOYSA-N
• Gene Information: human ... FNTA(2339)

Description

General description

Phenanthroline is a heterocyclic organic compound, which forms complexes with metal ions. These complexes have wide applications in biochemistry, catalysis and analytical chemistry. It complexes with iron and is exploited as a redox indicator.

Application

1,10-Phenanthroline monohydrate has been used:

• as an inhibitor of metalloproteinase (MMP) in MDA-MB-231 breast cancer cells
• as a negative control in in situ zymography of mice heart samples
• in in vitro resveratrol chelation and reducing activities assays to chelate iron

When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions.

Biochem/physiol Actions

Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H301,H410
• Precautionary Statements: P264 - P273 - P301 + P310 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves