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I5500

Sigma-Aldrich

Insulin from bovine pancreas

Insulin from bovine pancreas
1 of 1 reviewers received a sample product or took part in a promotion

≥25 USP units/mg (HPLC), powder

Synonym(s):

Insulin (cattle)

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About This Item

Empirical Formula (Hill Notation):
C254H377N65O75S6
CAS Number:
11070-73-8
Molecular Weight:
5733.49
EC Number:
234-291-2
MDL number:
MFCD00131380
UNSPSC Code:
51183600
NACRES:
NA.31
form:
powder
Pricing and availability is not currently available.

form

powder

Quality Level

200

specific activity

≥25 USP units/mg (HPLC)

impurities

≤0.5 EU/mg endotoxin

solubility

water: 2 mg/mL, clear, colorless (pH: 2)

cation traces

Zn: ≤1.0%

UniProt accession no.

P01317

shipped in

ambient

storage temp.

−20°C

InChI key

IXIBAKNTJSCKJM-BUBXBXGNSA-N

Gene Information

cow ... INS(280829)

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Related Categories

Application


  • Cross-frontal mode: An alternative methodology for Taylor dispersion analysis of monomodal sample.: This research presents a novel methodology for Taylor dispersion analysis, which could be applied to the study of insulin dispersion and its implications in biochemical research (Gouyon et al., 2023).

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Other Notes

InChI: 1S/C254H377N65O75S6/c1-29-131(24)205(312-192(334)103-256)250(389)317-204(130(22)23)246(385)287-158(74-81-199(344)345)216(355)281-155(70-77-188(260)330)220(359)307-183-116-399-400-117-184-242(381)305-177(110-321)238(377)293-161(87-122(6)7)223(362)294-167(94-139-52-60-145(324)61-53-139)226(365)282-153(68-75-186(258)328)217(356)289-160(86-121(4)5)221(360)284-157(73-80-198(342)343)219(358)301-173(100-189(261)331)233(372)297-169(96-141-56-64-147(326)65-57-141)229(368)308-182(241(380)303-175(253(393)394)102-191(263)333)115-398-396-113-180(212(351)270-106-193(335)277-152(71-78-196(338)339)215(354)280-150(50-41-83-268-254(264)265)210(349)269-107-194(336)278-165(92-137-45-35-31-36-46-137)225(364)296-166(93-138-47-37-32-38-48-138)228(367)298-170(97-142-58-66-148(327)67-59-142)236(375)318-206(135(28)323)251(390)319-84-42-51-185(319)244(383)285-151(49-39-40-82-255)213(352)276-134(27)252(391)392)310-248(387)202(128(18)19)315-234(373)163(89-124(10)11)291-227(366)168(95-140-54-62-146(325)63-55-140)295-222(361)159(85-120(2)3)288-207(346)132(25)274-214(353)156(72-79-197(340)341)286-245(384)201(127(16)17)314-235(374)164(90-125(12)13)292-231(370)172(99-144-105-267-119-273-144)300-237(376)176(109-320)279-195(337)108-271-211(350)179(112-395-397-114-181(309-243(183)382)240(379)275-133(26)208(347)304-178(111-322)239(378)316-203(129(20)21)249(388)311-184)306-224(363)162(88-123(8)9)290-230(369)171(98-143-104-266-118-272-143)299-218(357)154(69-76-187(259)329)283-232(371)174(101-190(262)332)302-247(386)200(126(14)15)313-209(348)149(257)91-136-43-33-30-34-44-136/h30-38,43-48,52-67,104-105,118-135,149-185,200-206,320-327H,29,39-42,49-51,68-103,106-117,255-257H2,1-28H3,(H2,258,328)(H2,259,329)(H2,260,330)(H2,261,331)(H2,262,332)(H2,263,333)(H,266,272)(H,267,273)(H,269,349)(H,270,351)(H,271,350)(H,274,353)(H,275,379)(H,276,352)(H,277,335)(H,278,336)(H,279,337)(H,280,354)(H,281,355)(H,282,365)(H,283,371)(H,284,360)(H,285,383)(H,286,384)(H,287,385)(H,288,346)(H,289,356)(H,290,369)(H,291,366)(H,292,370)(H,293,377)(H,294,362)(H,295,361)(H,296,364)(H,297,372)(H,298,367)(H,299,357)(H,300,376)(H,301,358)(H,302,386)(H,303,380)(H,304,347)(H,305,381)(H,306,363)(H,307,359)(H,308,368)(H,309,382)(H,310,387)(H,311,388)(H,312,334)(H,313,348)(H,314,374)(H,315,373)(H,316,378)(H,317,389)(H,318,375)(H,338,339)(H,340,341)(H,342,343)(H,344,345)(H,391,392)(H,393,394)(H4,264,265,268)/t131-,132-,133-,134-,135+,149-,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,200-,201-,202-,203-,204-,205-,206-/m0/s1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000396263
0000396263
0000393335
0000341280
0000324246

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Mohamed R Ben Khedher et al.
PeerJ, 6, e4166-e4166 (2018-01-16)
Diet-induced obese (DIO) mice were treated for five weeks with sage methanol extract (100 and 400 mg kg After two weeks of treatment, the lower dose of sage MetOH extract decreased blood glucose and plasma insulin levels during an oral glucose
Denise Laskowski et al.
Molecular reproduction and development, 85(7), 599-611 (2018-05-11)
Insulin functions as a regulator of metabolism and plays an important role in reproduction. Hyperinsulinemia is often observed in patients with obesity and diabetes type 2 and is known to impair fertility, but the underlying molecular mechanisms are only partly
Felix Weis et al.
Nature communications, 9(1), 4420-4420 (2018-10-26)
Understanding the structural biology of the insulin receptor and how it signals is of key importance in the development of insulin analogs to treat diabetes. We report here a cryo-electron microscopy structure of a single insulin bound to a physiologically
Anisha M Hammer et al.
Neoplasia (New York, N.Y.), 19(6), 496-508 (2017-05-16)
The extracellular matrix (ECM) is critical for mammary ductal development and differentiation, but how mammary fibroblasts regulate ECM remodeling remains to be elucidated. Herein, we used a mouse genetic model to activate platelet derived growth factor receptor-alpha (PDGFRα) specifically in
O Sharabi et al.
Endocrinology, 157(2), 928-941 (2015-12-19)
Sexual differentiation and maintenance of masculinity in crustaceans has been suggested as being regulated by a single androgenic gland (AG) insulin-like peptide (IAG). However, downstream elements involved in the signaling cascade remain unknown. Here we identified and characterized a gene
View All Related Papers

Protocols

HPLC Analysis of Insulin Variants and Analogs

Chromatographic technique identifies insulin variants efficiently, aiding in pharmacokinetics.

HPLC Analysis of Insulin Variants and Analogs

Chromatographic technique identifies insulin variants efficiently, aiding in pharmacokinetics.

HPLC Analysis of Insulin Variants and Analogs

Chromatographic technique identifies insulin variants efficiently, aiding in pharmacokinetics.

HPLC Analysis of Insulin Variants and Analogs

Chromatographic technique identifies insulin variants efficiently, aiding in pharmacokinetics.

1 Review This action will navigate to reviews.

Questions

1–10 of 11 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How do you determine the potency that is given in the CoA. Is the material qualified against another Insulin bovine standard (primary standard, e.g. Insulin (bovine) CRS)?

    1 answer

    1. The potency of this product is determined by the USP HPLC method and calculated based on the HPLC area %. The material is tested against the USP human insulin standard, product 1342106.

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  4. How should stock solutions of item I5500, Insulin from bovine pancreas be stored?

    1 answer

    1. Stock vials of bovine insulin should be stored at -20°C in a non-frost-free freeze.

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  5. Is Insulin product (I5500) a lyophilization powder?

    1 answer

    1. Yes, this product is lyophilized

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  6. What can be used to make a solution of insulin?

    1 answer

    1. Insulin is soluble in dilute acids and alkalis. The solubility of insulin is tested at 2 mg/mL in water (acidified to pH 2 with hydrochloric acid). One can also use 1 mL of water acidified to pH 2 with 10 μL of glacial acetic acid to dissolve 2 mg of insulin. A 10 mg/mL solution of bovine insulin in 25 mM HEPES at pH 8.2 is offered as Product No. I0516.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. What is the amino acid sequence for insulin?

    1 answer

    1. The sequence and structure of insulin can be found on the product information sheet (under Documents, above).

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  9. How many units of insulin are present in my vial of Product I5500, Insulin from bovine pancreas?

    1 answer

    1. Product No. I5500 has a specification of not less than 27 USP units/mg.  The exact value for the product received can be found the on lot-specific Certificate of Analysis.

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  10. What is the comparison between International Units (IU) and USP units for insulin?

    1 answer

    1. The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.

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1–10 of 11 Questions  

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

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    • Review 1
    • Vote 1
    5 out of 5 stars.

    Good

    Performed as expected. No problems whatsoever. Fine.

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