Skip to Content
Merck
All Photos(2)

Key Documents

MABF1032

Sigma-Aldrich

Anti-NKG2D (CD314) Antibody, clone 1D11

clone 1D11, from mouse

Synonym(s):

NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, CD314

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1D11, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... KLRK1(22914)

General description

NKG2-D type II integral membrane protein (UniProt: P26718; also known as Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, CD314) is encoded by the KLRK1 (also known as D12S2489E, NKG2D) gene (Gene ID: 100528032) in human. NK cell receptor D is a homodimeric, disulfide-linked, single-pass type II membrane protein that can also form heterohexamers along with four subunits of HCST/DAP10. NK cell receptor D contains a cytoplasmic domain (aa 1-51), a transmembrane helical domain (aa 52-72), and an extracellular domain (aa 73-216). It functions as an activating and co-stimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and co-stimulatory innate immune responses on activated killer (NK) cells, leading to their cytotoxic activity. Also acts as a co-stimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. NK cell receptor D is expressed in natural killer (NK) cells, CD8+ alpha-beta and gamma-delta T-cells. Its expression in NK cells is upregulated by interleukin 15 (IL15).

Specificity

Clone 1D11 detects NKG2-D type II integral membrane protein in human cells.

Immunogen

Whole NKL cells (aggressive cell line derived from human natural killer cell leukemia).

Application

Anti-NKG2D (CD314), clone 1D11, Cat. No. MABF1032, is a mouse monoclonal antibody that detects human NKG2-D type II integral membrane protein and has been tested for use in Flow Cytometry, Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry, Immunoprecipitation, ECM Adhesion and Inhibit
Fluorescence Activated Cell Sorting (FACS): A representative lot detected NKG2D (CD314) in Fluorescence Activated Cell Sorting applications (Groh, V., et. al. (2003). Proc Natl Acad Sci U S A. 100(16):9452-7).

Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected NKG2D (CD314) in CD3-/CD56+ T cells (Courtesy of Alejandro Lopez-Soto, Ph.D., Tumor Immunology Group, Universidad de Oviedo, IUOPA Spain).

Inhibits Activity/Function: A representative lot of Anti-NKG2D (CD314), clone 1D11, reduced the percentage of degranulating NK cells in freshly isolated PBMCs when co-incubated with K562 cells (Courtesy of Alejandro Lopez-Soto, Ph.D., Tumor Immunology Group, Universidad de Oviedo, IUOPA Spain).

Immunohistochemistry Analysis: A representative lot detected NKG2D (CD314) in Immunohistochemistry applications (Groh, V., et. al. (2003). Proc Natl Acad Sci U S A. 100(16):9452-7).

Inhibition Analysis: A representative lot inhibited NKG2D activated cytolytic responses of gamma delta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. (Bauer, S., et. al. (1999). Science. 285(5428):727-9).

Stimulates ECM Adhesion: A representative lot of Anti-NKG2D (CD314), clone 1D11 stimulated ECM Adhesion (Abeyweera, T.P., et. al. (2011). J Cell Biol. 192(4):675-90).

Flow Cytometry Analysis: A representative lot detected NKG2D (CD314) in Flow Cytometry applications (Wu, J., et. al. (2002). J Immunol. 169(3):1236-40; Bauer, S., et. al. (1999). Science. 285(5428):727-9; Wu, J., et. al. (1999). Science. 285(5428):730-2; Roberts, A.I., et. al. (2001). J Immunol. 167(10):5527-30).

Immunoprecipitation Analysis: A representative lot detected NKG2D (CD314) in Immunoprecipitation applications (Bauer, S., et. al. (1999). Science. 285(5428):727-9).

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Target description

25.27 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service