MAB3120
Anti-Cre Recombinase Antibody, clone 2D8
ascites fluid, clone 2D8, Chemicon®
Synonym(s):
Anti-Cre Antibody
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
![Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 clone 1.2B6, from mouse](/deepweb/assets/sigmaaldrich/product/images/224/939/8a711839-25ef-4e45-badd-208503950c8a/640/8a711839-25ef-4e45-badd-208503950c8a.jpg)
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2D8, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
Escherichia coli ... Cre(2777477)
Related Categories
General description
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. Anti-Cre Recombinase Antibody can be used to determine the success of gene manipulations in transgenic animals.
Specificity
Cre recombinase. The epitope is located between aa 77 and 343.
Immunogen
Cre recombinase (aa 77 to 343) fusion protein
Application
Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.
Immunofluorescence:
A previous lot of this antibody was used on IF.
Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.
Immunofluorescence:
A previous lot of this antibody was used on IF.
Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
Target description
38 kDa
Physical form
Ascites fluid. Contains no preservative
Unpurified
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
HeLa Nuclear Extract
HeLa Nuclear Extract
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Hong-Xiang Liu et al.
Developmental biology, 368(2), 294-303 (2012-06-05)
The epithelium of mammalian tongue hosts most of the taste buds that transduce gustatory stimuli into neural signals. In the field of taste biology, taste bud cells have been described as arising from "local epithelium", in distinction from many other
Extrasynaptic GABAA receptors in mediodorsal thalamic nucleus modulate fear extinction learning.
Paydar, A; Lee, B; Gangadharan, G; Lee, S; Hwang, EM; Shin, HS
Molecular Brain null
Axon position within the corpus callosum determines contralateral cortical projection.
Zhou, J; Wen, Y; She, L; Sui, YN; Liu, L; Richards, LJ; Poo, MM
Proceedings of the National Academy of Sciences of the USA null
Takashi Umehara et al.
Endocrinology, 157(12), 4899-4913 (2016-10-13)
Adult Leydig cells are derived from proliferating stem/progenitor Leydig cells in the infant testis and subsequent differentiation to steroidogenic cells in adult mice. Leydig cell proliferation in the infant testis occurs primarily in response to increased levels of LH that
Ja Wook Koo et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 39(11), 2646-2653 (2014-05-24)
The nucleus accumbens (NAc) has a central role in the mechanism of action of drugs of abuse. The major neuronal type within the NAc is the GABAergic medium spiny neuron (MSN), with two major subpopulations defined-termed D1-type and D2-type MSNs-based
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service