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MAB2022

Sigma-Aldrich

Anti-Keratan Sulfate Antibody, clone EFG-11

ascites fluid, clone EFG-11, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

clone

EFG-11, monoclonal

species reactivity

sheep, bovine, pig, canine, mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... LUM(4060)

Specificity

Recognizes 2-3 disaccharide units on both skeletal (type II) and corneal (type I) keratan sulfate chains either in isolated or proteoglycan-bound form. The epitope is resistant to proteolytic or alkaline cleavages and partially sensitive to keratanase or other endo-B-galactosidase treatment.

Immunogen

Human articular cartilage proteoglycan digested with chondroitinase ABC.

Application

Anti-Keratan Sulfate Antibody, clone EFG-11 is an antibody against Keratan Sulfate for use in ELISA, RIA & WB.
Research Category
Cell Structure
Research Sub Category
Cytokeratins
Western blot at 1:1000 - 1:4000

ELISA at 1:5,000-1:20,000

RIA at 1:30,000-1:500,000

May be used in a competitive inhibition assay to measure keratan sulfate levels.

Physical form

Ascites liquid, no preservative.

Storage and Stability

Maintain at -20°C in convenient aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jesús-José Ferre-Fernández et al.
Human genomics, 16(1), 49-49 (2022-10-27)
FOXC1 encodes a forkhead-domain transcription factor associated with several ocular disorders. Correct FOXC1 dosage is critical to normal development, yet the mechanisms controlling its expression remain unknown. Together with FOXQ1 and FOXF2, FOXC1 is part of a cluster of FOX
Monoclonal antibodies to different protein-related epitopes of human articular cartilage proteoglycans.
Glant, T T, et al.
The Biochemical Journal, 234, 31-41 (1986)
G Cs-Szabó et al.
Arthritis and rheumatism, 38(5), 660-668 (1995-05-01)
To identify characteristic changes in large aggregating (aggrecan) and small proteoglycan (PG) populations in articular cartilages during osteoarthritis (OA) and rheumatoid arthritis (RA). Aggrecan populations in guanidine extracts of femoral condylar cartilages of 46 OA and 8 RA patients who
Jerome Carpenter et al.
Glycobiology, 31(4), 436-443 (2020-10-22)
Understanding the basic elements of the airway mucosal surfaces and how they form a functional barrier is essential in understanding disease initiation, progression, pathogenesis and ultimately treating chronic lung diseases. Using primary airway epithelial cell cultures, atomic force microscopy (AFM)
P J Roughley et al.
Pediatric research, 22(4), 409-413 (1987-10-01)
Proteoglycan was isolated from the epiphyseal cartilage of the knee joint from human fetuses, ranging in age from 11 to 19 wk of gestation. The content of proteoglycan, per wet weight of cartilage, increased with gestational age, and structural changes

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