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201545

Sigma-Aldrich

Fast Blue RR

Synonym(s):

4-Benzoylamino-2,5-dimethoxyaniline, Azoic Diazo No. 24

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About This Item

Empirical Formula (Hill Notation):
C15H16N2O3
CAS Number:
6268-05-9
Molecular Weight:
272.30
Colour Index Number:
37155
EC Number:
228-441-6
MDL number:
MFCD00008369
UNSPSC Code:
12171500
PubChem Substance ID:
24852010
NACRES:
NA.47

Assay

>96.5% (HPLC)

Quality Level

100

form

solid

mp

165-168 °C (lit.)

λmax

323 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

COc1cc(NC(=O)c2ccccc2)c(OC)cc1N

InChI

1S/C15H16N2O3/c1-19-13-9-12(14(20-2)8-11(13)16)17-15(18)10-6-4-3-5-7-10/h3-9H,16H2,1-2H3,(H,17,18)

InChI key

DDRCIGNRLHTTIW-UHFFFAOYSA-N

Application

Fast Blue RR has been used to detect alkaline phosphatase activity.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appear black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Claude Reiss, Simone Parvez, Gilles Labbe
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The posterior parietal cortex (PPC) is a central hub for the primate forebrain networks that control skilled manual behavior, including tool use. Here, we quantified and compared the sources of thalamic input to electrophysiologically-identified hand/forearm-related regions of several PPC areas
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Chung TH
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C. F. A. Culling
Handbook of Histopathological and Histochemical Techniques (2013)
Jutta Schade et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(2), 147-155 (2007-10-31)
The expression of dipeptidyl peptidase 4 (DP4, CD26) affects T-cell recruitment to lungs in an experimental rat asthma model. Furthermore, the gene of the structural homologous DP10 represents a susceptibility locus for asthma in humans, and the functional homologous DP8/9

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