Exploring the human mesenchymal stem cell tubule communication network through electron microscopy.

Exploring the human mesenchymal stem cell tubule communication network through electron microscopy.

Ultrastructural pathology (2014-10-01)

Sabrina Valente, Roberta Rossi, Leonardo Resta, Gianandrea Pasquinelli

摘要

Cells use several mechanisms to transfer information to other cells. In this study, we describe micro/nanotubular connections and exosome-like tubule fragments in multipotent mesenchymal stem cells (MSCs) from human arteries. Scanning and transmission electron microscopy allowed characterization of sinusoidal microtubular projections (700 nm average size, 200 µm average length, with bulging mitochondria and actin microfilaments); short, uniform, variously shaped nanotubular projections (100 nm, bidirectional communication); and tubule fragments (50 nm). This is the first study demonstrating that MSCs from human arteries constitutively interact through an articulate and dynamic tubule network allowing long-range cell to cell communication.

材料

產品編號

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產品描述

E7023

Sigma-Aldrich

纯乙醇, 200 proof, for molecular biology

459844

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纯乙醇, 200 proof, ACS reagent, ≥99.5%

459828

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纯乙醇, 200 proof, HPLC/spectrophotometric grade

493546

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纯乙醇, 200 proof, meets USP testing specifications

E7148

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纯乙醇, 190 proof, for molecular biology

440191

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六甲基二硅氮烷, reagent grade, ≥99%

02870

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酒精, ACS reagent, prima fine spirit, without additive, F15 o¹

02856

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酒精, purum, absolute ethanol, denaturated with 4.8% isopropanol, A15 IPA¹, ≥99.8% (based on denaturant-free substance)

459836

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纯乙醇, 200 proof, anhydrous, ≥99.5%

51976

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酒精, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)

128910

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N,O-双(三甲基甲硅烷基)乙酰胺, synthesis grade, ≥95%

379212

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六甲基二硅氮烷, ReagentPlus^®, 99.9%

22874

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活性炭 Norit^®, Norit^® PK 1-3, from peat, steam activated, granular

493511

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719781

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碳纳米纤维, graphitized (iron-free), composed of conical platelets, D × L 100 nm × 20-200 μm

02857

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719803

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53663

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93067

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699624

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Supelco

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493538

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02890

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