Surface characterization and osteoblast response to a functionally graded hydroxyapatite/fluoro-hydroxyapatite/titanium oxide coating on titanium surface by sol-gel method.

Surface characterization and osteoblast response to a functionally graded hydroxyapatite/fluoro-hydroxyapatite/titanium oxide coating on titanium surface by sol-gel method.

Cell proliferation (2014-04-18)

G He, B Guo, H Wang, C Liang, L Ye, Y Lin, X Cai

摘要

To improve efficacy of current titanium and its alloys, in bioactivity and speed of osseointegration, of orthopaedic implants. A novel triple-layered functional graded coating, consisting of a porous hydroxyapatite (HA) outermost layer, fluoro-HA (FHA) intermediate layer and titanium oxide (TiO2 ) innermost layer, was created on a titanium substrate by a multistep sol-gel method. X-ray diffraction analysis showed TiO2 anatase and apatite crystallization in the coating. Morphological analysis performed by scanning electron microscopy showed excellent bonding between coating and substrate, with a thickness of ~2 μm. Scratch testing found favourable adhesion strength of the composite coating. In addition, optical microscope images suggested good biocompatibility. Considering thet in vitro cell response, osteoblasts on the coating exhibited higher cell proliferation and ALP activity compared to pure titanium and HA coating, and demonstrated excellent coating bioactivity. Current results indicated that the novel TiO2 /FHA/HA coating has promising clinical applications in orthopaedic and dental implantation.

材料

產品編號

品牌

產品描述

718467

Sigma-Aldrich

氧化钛（IV）, nanopowder, 21 nm primary particle size (TEM), ≥99.5% trace metals basis

637254

Sigma-Aldrich

氧化钛 (IV)，锐钛矿, nanopowder, <25 nm particle size, 99.7% trace metals basis

14027

Sigma-Aldrich

氧化钛（IV）, puriss., meets analytical specification of Ph. Eur., BP, USP, 99-100.5%

232033

Sigma-Aldrich

氧化钛 (IV)，锐钛矿, powder, 99.8% trace metals basis

14021

Sigma-Aldrich

氧化钛（IV）, ReagentPlus^®, ≥99%

224227

Sigma-Aldrich

钛 (IV) 氧化物，金红石型, powder, <5 μm, ≥99.9% trace metals basis

248576

Sigma-Aldrich

氧化钛 (IV)，锐钛矿, powder, −325 mesh, ≥99% trace metals basis

637262

Sigma-Aldrich

钛 (IV) 氧化物，金红石型, nanopowder, <100 nm particle size, 99.5% trace metals basis

634662

Sigma-Aldrich

氧化钛(IV)，金红石和锐钛矿混合物, nanopowder, <100 nm particle size (BET), 99.5% trace metals basis

348791

Sigma-Aldrich

钛, foil, thickness 0.127 mm, 99.7% trace metals basis

366994

Sigma-Aldrich

钛, powder, <45 μm avg. part. size, 99.98% trace metals basis

267503

Sigma-Aldrich

钛, foil, thickness 0.25 mm, 99.7% trace metals basis

700347

Sigma-Aldrich

氧化钛(IV)，金红石和锐钛矿混合物, nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H₂O, 99.5% trace metals basis

268496

Sigma-Aldrich

钛, powder, −100 mesh, 99.7% trace metals basis

204757

Sigma-Aldrich

钛 (IV) 氧化物，金红石型, ≥99.98% trace metals basis

204730

Sigma-Aldrich

钛 (IV) 氧化物，金红石型, 99.995% trace metals basis

677469

Sigma-Aldrich

氧化钛（IV）, contains 1% Mn as dopant, nanopowder, <100 nm particle size (BET), ≥97%

268526

Sigma-Aldrich

钛, sponge, 1-20 mm, 99.5% trace metals basis

460400

Sigma-Aldrich

钛, wire, diam. 0.25 mm, 99.7% trace metals basis

774510

Sigma-Aldrich

氧化钛（IV）, nanowires, diam. × L ~100 nm × 10 μm

369489

Sigma-Aldrich

钛, foil, thickness 2.0 mm, 99.7% trace metals basis

348848

Sigma-Aldrich

钛, foil, thickness 0.025 mm, 99.98% trace metals basis

774529

Sigma-Aldrich

氧化钛（IV）, nanowires, diam. × L ~10 nm × 10 μm

348805

Sigma-Aldrich

钛, foil, thickness 0.5 mm, 99.99% trace metals basis

767506

Sigma-Aldrich

钛, sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.995% trace metals basis

460397

Sigma-Aldrich

钛, foil, thickness 0.127 mm, ≥99.99% trace metals basis

266035

Sigma-Aldrich

钛, wire, diam. 1.0 mm, 99.99% trace metals basis

635057

Sigma-Aldrich

钛 (IV) 氧化物，金红石型, <001>, (single crystal substrate), ≥99.9% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

267481

Sigma-Aldrich

钛, foil, thickness 0.25 mm, 99.99% trace metals basis

04238

Sigma-Aldrich

羟基磷灰石, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)