Capillary electrophoretic separation of TNT and its transformation products.

2,4,6-Trinitrotoluene (TNT), 2-aminodinitrotoluene (2ADNT), 4-aminodinitrotoluene (4ADNT), and diamino-6-nitrotoluene (DA6NT) in a variety of matrices (including chemical, microbial, and plant extract) were successfully separated and quantified using micellar electrokinetic capillary electrophoresis (MEKC). The method used a buffer solution of 20 mM in borax, 50 mM in sodium dodecyl sulfate (SDS), and of pH 8.5. Voltage applied across the capillary was 20 kV and temperature was maintained at 25 degrees C. Compounds eluted within approximately 6-11 min and were identified by both their migration times and spectra.