跳轉至內容
Merck
  • The effect of long term storage on tobacco smoke particulate matter in in vitro genotoxicity and cytotoxicity assays.

The effect of long term storage on tobacco smoke particulate matter in in vitro genotoxicity and cytotoxicity assays.

Regulatory toxicology and pharmacology : RTP (2012-12-12)
I Crooks, D M Dillon, J K Scott, M Ballantyne, C Meredith
摘要

Particulate matter (PM) collected from mainstream tobacco smoke is a test article commonly used for in vitro genotoxicity and cytotoxicity testing of combustible tobacco products. However, little published data exists concerning the stability of PM. We completed a 2 year study to quantify the effect of PM storage at -80 °C, on the genotoxicity and cytotoxicity of PM generated from 3R4F and M4A reference cigarettes. The Ames test, Micronucleus assay (MNvit), Mouse Lymphoma assay (MLA) and the Neutral Red Uptake assay (NRU) were used. The majority of M4A and 3R4F PMs were genotoxic and cytotoxic at the timepoints tested. Some minor but statistically significant differences were observed for stored versus freshly prepared PM, but the magnitude of changes were within the variability observed for repeat testing.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
中性红溶液 (0.33%), 3.3 g/L in DPBS, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
中性红, Dye content ≥90 %
Sigma-Aldrich
中性红, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
中性红, for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0)
Sigma-Aldrich
中性红 溶液
Sigma-Aldrich
中性红, certified by the Biological Stain Commission