Direct red 81 and amido black stain proteins in polyacrylamide electrophoresis gels within 10 min.

Direct red 81 and amido black stain proteins in polyacrylamide electrophoresis gels within 10 min.

Analytical biochemistry (2010-01-12)

Ikechukwu Achilonu, J P Dean Goldring

PMID20060376

摘要

Proteins separated by SDS-polyacrylamide gel electrophoresis can be stained with organic dyes, the most popular being Coomassie brilliant blue R-250. Coomassie R-250 staining of ovalbumin in an SDS-PAGE gel increased linearly from 2.5 to 60 min. Direct red 81 and amido black staining approached saturation in 10 min. Scatchard analysis showed that the number of direct red 81 and amido black ligands bound to ovalbumin was fourfold higher than that of Coomassie R-250. Direct red 81 and amido black stain proteins in an SDS-polyacrylamide electrophoresis gel in 10 min.

材料

產品編號

品牌

產品描述

A8181

Sigma-Aldrich

氨基黑染色液 2 X, electrophoresis reagent

N3393

Sigma-Aldrich

萘酚蓝黑, BioReagent, suitable for electrophoresis

195243

Sigma-Aldrich

萘酚蓝黑, Dye content 80 %

195251

Sigma-Aldrich

Direct Red 81, Dye content 50 %

N9002

Sigma-Aldrich

萘酚蓝黑, Dye content ~50 %