Silyl protecting groups for oligonucleotide synthesis removed by a ZnBr2 treatment.

Silyl protecting groups for oligonucleotide synthesis removed by a ZnBr2 treatment.

Nucleosides, nucleotides & nucleic acids (2005-10-27)

Fernando Ferreira, François Morvan

摘要

An oligonucleotide protected with N-(trimethylsilyloxycarbonyl) (Teoc) and P-(trimethylsilylethanol) (Tse) groups was synthesized and deprotected by a single ZnBr2 treatment. Finally it was released from the solid support by cleavage of a disulfide linkage with TCEP. The olgonucleotide was obtained without any basic treatment.

材料

產品編號

品牌

產品描述

230022

Sigma-Aldrich

溴化锌, 99.999% trace metals basis

451398

Sigma-Aldrich

溴化锌, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis