Replacement of cyanogen bromide solution PH. EUR. with 1,3-dibromo-5,5-dimethylhydantoin (DBH). Analytical methods of pharmacopoeias with DBH in respect to environmental and economical concern, Part 13.

PH. EUR. 2002 identifies nicotinamid, nicotinic acid, and nikethamide according the reaction of König using cyanogen bromide solution prepared with bromine water and ammonium thiocyanate immediately before use. This colour reaction can be better performed with 1,3-dibromo-5,5-dimethylhydantoin (DBH) and sulphanilic acid as condensation component. The prescriptions of PH. EUR. have been improved in respect to environmental and economical concern.