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Merck
  • Use of detergent extracts of Brucella abortus RB51 to detect serologic responses in RB51-vaccinated cattle.

Use of detergent extracts of Brucella abortus RB51 to detect serologic responses in RB51-vaccinated cattle.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (2001-10-03)
S M Halling, N A Koster
摘要

Serologic responses to the newly introduced rough Brucella abortus vaccine strain RB51 have been determined in a dot-blot format using gamma-irradiated RB51 cells as the antigen. Because gamma-irradiated cells are not easily prepared and the signal from cells was not always reliable, an alternative antigen was sought. Detergent extracts of B. abortus RB51 were prepared using zwittergent 3-14, Triton X-100, and sodium dodecyl sulfate (SDS) and examined in a dot-blot format. Zwittergent 3-14 extracts and gamma-irradiated RB51 cells gave the same titers. Unlike gamma-irradiated RB51 cells, zwittergent 3-14 extracts produced signals consistently, and the signals were easily interpreted. Triton X-100 extracts interfered with signal development, and SDS extracts resulted in a high background signal. Western blot analyses revealed several outer membrane proteins in the zwittergent 3-14 extract. The major antigens in the extract had apparent molecular weights of <20,000.

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Sigma-Aldrich
3-( N , N -二甲基肉豆蔻铵基)丙磺酸盐, ≥99% (TLC)
Sigma-Aldrich
3-( N , N -二甲基肉豆蔻铵基)丙磺酸盐, ≥99% (TLC), BioXtra
Sigma-Aldrich
3-( N , N -二甲基肉豆蔻铵基)丙磺酸盐, ≥98.0% (TLC)