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  • Aging and sequential resistance exercise bout effects on housekeeping gene messenger RNA expression in human skeletal muscle.

Aging and sequential resistance exercise bout effects on housekeeping gene messenger RNA expression in human skeletal muscle.

Journal of strength and conditioning research (2012-10-23)
Kyle L Sunderland, Michael D Roberts, Vincent J Dalbo, Chad M Kerksick
摘要

The purpose of this study was to investigate how age and 1 week of conventional resistance exercise affects commonly used housekeeping gene (HKG) messenger RNAs (mRNAs) in skeletal muscle. Ten college-aged (18-25 years) and 10 older (60-76 years) men completed 3 lower-body resistance exercise bouts on Monday, Wednesday, and Friday, and muscle samples were obtained before bout 1 (T1), 48 hours after the first (T2) and second bouts (T3), and 24 hours after the third bout (T4). Raw Ct values indicated that β-actin and cyclophilin were more highly expressed in older vs. younger males (p < 0.01) at T1. When normalizing each HKG mRNA to the other 4 HKG mRNAs, CYC increased at T3 and glyceraldehyde-3-phosphate dehydrogenase decreased at T2 (p < 0.05) in younger men. This is one of the few studies to suggest that explicit HKG mRNAs should be used depending upon age group and resistance exercise intervention.

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Sigma-Aldrich
甘油醛-3-磷酸脱氢酶 来源于兔肌肉, lyophilized powder, ≥75 units/mg protein
Supelco
GAPDH, standard for protein electrophoresis
Sigma-Aldrich
甘油醛-3-磷酸脱氢酶 来源于人类红细胞, lyophilized powder, 50-150 units/mg protein