跳轉至內容
Merck
  • SIRT1 promotes lipid metabolism and mitochondrial biogenesis in adipocytes and coordinates adipogenesis by targeting key enzymatic pathways.

SIRT1 promotes lipid metabolism and mitochondrial biogenesis in adipocytes and coordinates adipogenesis by targeting key enzymatic pathways.

Scientific reports (2021-04-16)
Yasser Majeed, Najeeb Halabi, Aisha Y Madani, Rudolf Engelke, Aditya M Bhagwat, Houari Abdesselem, Maha V Agha, Muneera Vakayil, Raphael Courjaret, Neha Goswami, Hisham Ben Hamidane, Mohamed A Elrayess, Arash Rafii, Johannes Graumann, Frank Schmidt, Nayef A Mazloum
摘要

The NAD+-dependent deacetylase SIRT1 controls key metabolic functions by deacetylating target proteins and strategies that promote SIRT1 function such as SIRT1 overexpression or NAD+ boosters alleviate metabolic complications. We previously reported that SIRT1-depletion in 3T3-L1 preadipocytes led to C-Myc activation, adipocyte hyperplasia, and dysregulated adipocyte metabolism. Here, we characterized SIRT1-depleted adipocytes by quantitative mass spectrometry-based proteomics, gene-expression and biochemical analyses, and mitochondrial studies. We found that SIRT1 promoted mitochondrial biogenesis and respiration in adipocytes and expression of molecules like leptin, adiponectin, matrix metalloproteinases, lipocalin 2, and thyroid responsive protein was SIRT1-dependent. Independent validation of the proteomics dataset uncovered SIRT1-dependence of SREBF1c and PPARα signaling in adipocytes. SIRT1 promoted nicotinamide mononucleotide acetyltransferase 2 (NMNAT2) expression during 3T3-L1 differentiation and constitutively repressed NMNAT1 and 3 levels. Supplementing preadipocytes with the NAD+ booster nicotinamide mononucleotide (NMN) during differentiation increased expression levels of leptin, SIRT1, and PGC-1α and its transcriptional targets, and reduced levels of pro-fibrotic collagens (Col6A1 and Col6A3) in a SIRT1-dependent manner. Investigating the metabolic impact of the functional interaction of SIRT1 with SREBF1c and PPARα and insights into how NAD+ metabolism modulates adipocyte function could potentially lead to new avenues in developing therapeutics for obesity complications.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
胰岛素 溶液 人, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
地塞米松, ≥98% (HPLC), powder
Sigma-Aldrich
β-烟酰胺单核苷酸, ≥95% (HPLC)
Sigma-Aldrich
β-烟酰胺腺嘌呤二核苷酸, pkg of 10 mg (per vial)
Sigma-Aldrich
Anti-Leptin Antibody, Chemicon®, from rabbit