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  • HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation.

HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation.

Molecular plant (2020-02-23)
Chao Yang, Wenjin Shen, Lianming Yang, Yun Sun, Xibao Li, Minyi Lai, Juan Wei, Chaojun Wang, Yingchao Xu, Faqiang Li, Shan Liang, Chengwei Yang, Shangwei Zhong, Ming Luo, Caiji Gao
摘要

Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development, but the molecular link between light signaling and the autophagy pathway has not been elucidated in plants. In this study, we demonstrate that autophagy is activated during light-to-dark conversion though transcriptional upregulation of autophagy-related genes (ATGs). We showed that depletion of the ELONGATED HYPOCOTYL 5 (HY5), a key component of light signaling, leads to enhanced autophagy activity and resistance to extended darkness and nitrogen starvation treatments, contributing to higher expression of ATGs. HY5 interacts with and recruits HISTONE DEACETYLASE 9 (HDA9) to ATG5 and ATG8e loci to repress their expression by deacetylation of the Lys9 and Lys27 of histone 3. Furthermore, we found that both darkness and nitrogen depletion induce the degradation of HY5 via 26S proteasome and the concomitant disassociation of HDA9 from ATG5 and ATG8e loci, leading to their depression and thereby activated autophagy. Genetic analysis further confirmed that HY5 and HDA9 act synergistically and function upstream of the autophagy pathway. Collectively, our study unveils a previously unknown transcriptional and epigenetic network that regulates autophagy in response to light-to-dark conversion and nitrogen starvation in plants.

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Sigma-Aldrich
ChIPAb+ Acetyl-Histone H3 (Lys9) Purified - ChIP Validated Antibody and Primer Set, from rabbit, purified by using Protein A
Sigma-Aldrich
ChIPAb+ 乙酰化组蛋白H3(Lys27)- ChIP验证的抗体和引物组, clone CMA309, from mouse