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Merck

A molecular mechanism for the procentriole recruitment of Ana2.

The Journal of cell biology (2019-12-17)
Tiffany A McLamarrah, Sarah K Speed, John M Ryniawec, Daniel W Buster, Carey J Fagerstrom, Brian J Galletta, Nasser M Rusan, Gregory C Rogers
摘要

During centriole duplication, a preprocentriole forms at a single site on the mother centriole through a process that includes the hierarchical recruitment of a conserved set of proteins, including the Polo-like kinase 4 (Plk4), Ana2/STIL, and the cartwheel protein Sas6. Ana2/STIL is critical for procentriole assembly, and its recruitment is controlled by the kinase activity of Plk4, but how this works remains poorly understood. A structural motif called the G-box in the centriole outer wall protein Sas4 interacts with a short region in the N terminus of Ana2/STIL. Here, we show that binding of Ana2 to the Sas4 G-box enables hyperphosphorylation of the Ana2 N terminus by Plk4. Hyperphosphorylation increases the affinity of the Ana2-G-box interaction, and, consequently, promotes the accumulation of Ana2 at the procentriole to induce daughter centriole formation.

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胰蛋白酶抑制剂 来源于大豆, lyophilized powder
Sigma-Aldrich
Phosphatase Inhibitor Cocktail Set I, Phosphatase Inhibitor Cocktail Set I contains three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A.
Supelco
3,5,6-三氯-2-吡啶酚, PESTANAL®, analytical standard