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一般說明
用于全转录组扩增(WTA)的SeqPlex™RNA扩增试剂盒(SEQXE)专用于对少量或降解/高度片段化的RNA（如来自福尔马林固定石蜡包埋（FFPE）组织样本的RNA ）进行下一代测序(NGS)。通过SeqPlex试剂盒可对这类RNA以及其它用于NGS的少量/高度片段化RDNA样品进行预扩增。它还有助于扩增从组织、培养细胞、福尔马林固定样品或血清中分离的非 polyA 尾 RNA，同时保持未扩增样品中发现的差异表达模式。
特點和優勢
- 少量总 RNA 随机引物技术可扩增来自所有来源（包括 FFPE 和 RIP）的片段化或完整 RNA。
- 半简并文库引物设计，可实现更完整的转录组覆盖和高效引发。
- 测序前无需进行 DNA的片段化。
- 在 8 小时或更短的时间内扩增 ds-cDNA。
- 兼容Pacific Bioscience以外的其它所有下一代测序平台。
其他說明
SEQR-500RXN 按需生产。请联系我们获取更多信息。
法律資訊
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
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