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Key Documents

Z374911

PCR 多孔板

size 384 wells, polypropylene, skirt, non-sterile

同義詞:

384 multiwell PCR plate, 384 well PCR microplate, 384 well PCR plate, 384 well microtiter plate

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About This Item

分類程式碼代碼:
41106300
NACRES:
NB.22

材料

colorless polypropylene
polypropylene

無菌

non-sterile
non-sterile

特點

skirt

包裝

case of 50 ea

製造商/商標名

Sorenson 39620

技術

PCR: suitable

尺寸

384 wells

孔容積

40 μL

孔有效容積

25 μL

適合性

suitable for (PCR, RT-PCR or DNA purification applications)

應用

agriculture

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一般說明

384 well PCR plates are skirted for compatibility with automation systems. Wells have raised rims to ensure contact with sealing film and reduce evaporation. Each well has a capacity of 40 μL and a working volume of 25 μL.
刚性顶板(随附)可最大限度地减小板变形,确保与PCR热循环仪的可靠配合,并允许使用微垫子或帽条进行防漏密封。每孔最大容量为200μL。

特點和優勢

  • 原始聚丙烯
  • 完全可高压灭菌
  • 经认证的无DNasa和RNase
  • 微孔壁薄,可实现快速温度平衡并缩短周期

分析證明 (COA)

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Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.

Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.

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相關內容

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.

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