推薦產品
材料
colorless cap
colorless tube
conical bottom
flat cap
polypropylene
polypropylene cap
polypropylene tube
描述
Autoclavable
無菌
non-sterile
特點
RNase and DNase free
autoclavable at 121 °C
graduations: no
Push fit
包裝
pack of 250 ea
case of 4 packs
技術
PCR: suitable
容量
0.2 mL
顏色
clear
適合性
suitable for (PCR applications)
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一般說明
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Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.
Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
相關內容
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
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