推薦產品
生物源
Glycine max (soybean)
無菌
sterile-filtered
形狀
solution
分子量
20,100 Da
技術
inhibition assay: suitable
溶解度
balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble
運輸包裝
ambient
儲存溫度
2-8°C
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生化/生理作用
该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X,机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物,然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。
單位定義
一个胰蛋白酶单位是指在25°C、pH 7.6条件下,以N-α-苯甲酰基-L-精氨酸乙酯(BAEE)为底物,每分钟产生0.001的 A253所需的酶量。
在 pH 7.6 和 25°C 下以 BAEE 为底物,一单位胰蛋白酶每分钟产生的 ΔA253 为 0.001;其中反应体积为 3.2mL,光程为 1cm。
準備報告
由 I-S 型制备
胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊,呈黄色至琥珀色。使用胰蛋白酶消化细胞后,重悬于胰蛋白酶抑制剂溶液中,使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心,得到细胞沉淀。
在2-8℃下短期储存时,溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。
在2-8℃下短期储存时,溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。
分析報告
由缩二脲确定的蛋白质。
其他說明
查看更多关于 胰蛋白酶抑制剂的信息。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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