ABE484

Anti-LOXL2

from rabbit, purified by affinity chromatography

• 同義詞: Lysyl oxidase homolog 2, EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
• 包裝: antibody small pack of 25 μg
• 技術: immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_002309.1
• UniProt登錄號: Q9Y4K0
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... LOXL2(4017) ; mouse ... Loxl2(94352)

描述

一般說明

Lysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-1, LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 activity is strongly induced in hypoxia and it is reported to be a direct transcriptional target of HIF1A.

Lysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 activity is strongly induced in hypoxia and it is reported to be a direct transcriptional target of HIF1A.

特異性

This rabbit polyclonal antibody detects Lysyl oxidase homolog 2 in human cells. It targets an epitope within 16 amino acids from the N-terminal half.

免疫原

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Lysyl oxidase homolog 2.

應用

Anti-LOXL2, Cat. No. ABE484, is a highly specific rabbit polyclonal antibody that targets Lysyl oxidase homolog 2 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A 1:500 dilution from a representative lot detected LOXL2 in MDA-MB-231 cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LOXL2 in human prostate and human placenta tissues.
Immunoprecipitation Analysis: A representative lot detected LOXL2 in LoxL2 expressing vector (Courtesy Dr Sandra Peiro from the Institut Hospital del Mar d′Investigacions Mèdiques (IMIM) in Barcelona Spain ).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected LOXL2 in MDA-MB-231 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LOXL2 in human prostate and human placenta tissues.

Immunoprecipitation Analysis: A representative lot detected LOXL2 in LoxL2 expressing vector (Courtesy Dr Sandra Peiro from the Institut Hospital del Mar d′Investigacions Mèdiques (IMIM) in Barcelona Spain).

nti-LOXL2, Cat. No. ABE484, is a highly specific rabbit polyclonal antibody that targets Lysyl oxidase homolog 2 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

品質

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LOXL2 in A431 cell lysate.

標靶描述

~82 kDa obseerved; 86.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable