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16-638

Sigma-Aldrich

4G10® 铂,抗磷酸酪氨酸抗体,琼脂糖偶联物

clone 4G10®, Upstate®, from mouse

同義詞:

4G10 Platinum Anti-Phosphotyrosine, 4G10 Platinum Antibody, Phosphotyrosine Detection Antibody

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體產品種類

primary antibodies

無性繁殖

4G10®, monoclonal

物種活性(以同源性預測)

all

製造商/商標名

Upstate®

技術

immunoprecipitation (IP): suitable

同型

IgG2bκ

運輸包裝

wet ice

目標翻譯後修改

phosphorylation (pTyr)

基因資訊

human ... PID1(55022)

一般說明

1989年抗磷酸酪氨酸克隆4G10的开发对研究人员来说是一个巨大的发现。4G10是检测酪氨酸磷酸化的第一个也是最佳单克隆抗体。4G10以其灵敏度和检测多种底物上多种酪氨酸磷酸化的能力而闻名。在过去的20年中,它已经在几乎每个应用和酪氨酸靶标中得到了数千名科学和医学研究人员的验证。现在Millipore用4G10铂做到最好甚至更好。 我们将4G10 与下一个最受推崇的抗磷酸酪氨酸克隆PY20合并在一起,制成4G10 铂。PY20本身是4G10的非常差的替代品,但是它的累加效应允许在更多的底物上进行更高水平的检测,而即使单独使用4G10也能做到这一点。

免疫原

4G10的免疫原使用与KLH偶联的磷酸酪胺,PY20的免疫原使用与载体蛋白偶联的磷酸酪氨酸。

應用

4G10铂,抗磷酸酪氨酸,琼脂糖结合物可检测磷酸酪氨酸蛋白的水平&已发表&并经验证用于IP。

特點和優勢

形式:凝胶固定

品質

免疫沉淀:
使用20 μl该试剂的50%浆液(10 μl包装的珠子)从250 μg PDGF刺激的NIH3T3细胞裂解物或EGF刺激的A431细胞裂解物中免疫沉淀酪氨酸磷酸化的蛋白。

標靶描述

依赖于酪氨酸磷酸化蛋白的分子量

外觀

以琼脂糖偶联的,蛋白G纯化的小鼠单克隆4G10(IgG2bκ)和蛋白A纯化的PY20(IgG2b)的专有混合物的50%浆液形式提供,溶于PBS(pH 7.4)和0.05%叠氮化钠。
蛋白G纯化的小鼠单克隆4G10(IgG2bκ)和蛋白A纯化的PY20(IgG2b)

儲存和穩定性

自发货之日起在2-8°C下可稳定保存6个月。 为了最大程度地回收产品,在取下盖子之前,将样品瓶进行离心。
注:不可冷冻。

法律資訊

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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S Ruff-Jamison et al.
The Journal of biological chemistry, 266(10), 6607-6613 (1991-04-15)
Phosphotyrosine and similar analogs have been used to elicit antibodies that have found widespread use in the study of cellular tyrosine phosphorylation. In order to better understand the anti-phosphotyrosine immune response and to elucidate the details of the specific association
Rachit Badolia et al.
The Journal of biological chemistry, 292(35), 14516-14531 (2017-07-15)
Platelets play a key role in the physiological hemostasis or pathological process of thrombosis. Rhodocytin, an agonist of the C-type lectin-like receptor-2 (CLEC-2), elicits powerful platelet activation signals in conjunction with Src family kinases (SFKs), spleen tyrosine kinase (Syk), and
Claudia Voena et al.
Cancer research, 67(9), 4278-4286 (2007-05-08)
Anaplastic large cell lymphomas (ALCL) are mainly characterized by the reciprocal translocation t(2;5)(p23;q35) that involves the anaplastic lymphoma kinase (ALK) gene and generates the fusion protein NPM-ALK with intrinsic tyrosine kinase activity. NPM-ALK triggers several signaling cascades, leading to increased
Francesco E Boccalatte et al.
Blood, 113(12), 2776-2790 (2008-10-11)
Anaplastic large cell lymphoma represents a subset of neoplasms caused by translocations that juxtapose the anaplastic lymphoma kinase (ALK) to dimerization partners. The constitutive activation of ALK fusion proteins leads to cellular transformation through a complex signaling network. To elucidate
Philippe Foubert et al.
The Journal of clinical investigation, 117(6), 1527-1537 (2007-05-19)
Endothelial progenitor cell (EPC) transplantation has beneficial effects for therapeutic neovascularization; however, only a small proportion of injected cells home to the lesion and incorporate into the neocapillaries. Consequently, this type of cell therapy requires substantial improvement to be of

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