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![水，PCR 级 liquid, pkg of 25 mL (03315959001 [1 x 25 ml]), pkg of 25 mL (03315932001 [25 x 1 ml]), pkg of 100 mL (03315843001 [4 x 25 ml])](/deepweb/assets/sigmaaldrich/product/images/326/208/bac83eed-3c88-4738-b4b5-50d3b223dd1c/640/bac83eed-3c88-4738-b4b5-50d3b223dd1c.jpg)
product name
水, PCR Reagent
等級
PCR Reagent
品質等級
蒸汽密度
<1 (vs air)
蒸汽壓力
3 mmHg
無菌
sterile-filtered
形狀
liquid
包裝
vial of 1.5 mL
技術
PCR: suitable
折射率
n20/D 1.34 (lit.)
pH值
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
密度
1.000 g/mL at 3.98 °C (lit.)
異物活動
DNase, none detected
RNase, none detected
SMILES 字串
O
InChI
1S/H2O/h1H2
InChI 密鑰
XLYOFNOQVPJJNP-UHFFFAOYSA-N
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一般說明
應用
- 作为反应混合物的组分和微流控RT-qPCR的稀释剂
- 作为反应混合物的组分，以扩增真菌（杂色云芝）DNA的产物
- 作为稀释剂和反应混合物的组分，以扩增cDNA
- 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积
適合性
其他說明
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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文章
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條款
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
相關內容
Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
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