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  • The NF-YC-RGL2 module integrates GA and ABA signalling to regulate seed germination in Arabidopsis.

The NF-YC-RGL2 module integrates GA and ABA signalling to regulate seed germination in Arabidopsis.

Nature communications (2016-09-15)
Xu Liu, Pengwei Hu, Mingkun Huang, Yang Tang, Yuge Li, Ling Li, Xingliang Hou
PMID27624486
ABSTRACT

The antagonistic crosstalk between gibberellic acid (GA) and abscisic acid (ABA) plays a pivotal role in the modulation of seed germination. However, the molecular mechanism of such phytohormone interaction remains largely elusive. Here we show that three Arabidopsis NUCLEAR FACTOR-Y C (NF-YC) homologues NF-YC3, NF-YC4 and NF-YC9 redundantly modulate GA- and ABA-mediated seed germination. These NF-YCs interact with the DELLA protein RGL2, a key repressor of GA signalling. The NF-YC-RGL2 module targets ABI5, a gene encoding a core component of ABA signalling, via specific CCAAT elements and collectively regulates a set of GA- and ABA-responsive genes, thus controlling germination. These results suggest that the NF-YC-RGL2-ABI5 module integrates GA and ABA signalling pathways during seed germination.

MATERIALS
Product Number
Brand
Product Description
F3165
Sigma-Aldrich
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
16-201
Sigma-Aldrich
Protein G Agarose/Salmon Sperm DNA, 2.5 mL, for use in chromatin immunoprecipitations (ChIP assays)
IP10
Millipore
Protein G Plus/Protein A-Agarose, A mixture of Protein G PLUS and Protein A covalently conjugated to agarose. Useful for purification of IgG from biological fluids.