- Lumican: a new inhibitor of matrix metalloproteinase-14 activity.
Lumican: a new inhibitor of matrix metalloproteinase-14 activity.
FEBS letters (2014-10-12)
Katarzyna Pietraszek, Aurore Chatron-Colliet, Stéphane Brézillon, Corinne Perreau, Anna Jakubiak-Augustyn, Hubert Krotkiewski, François-Xavier Maquart, Yanusz Wegrowski
PMID25304424
ABSTRACT
We previously showed that lumican regulates MMP-14 expression. The aim of this study was to compare the effect of lumican and decorin on MMP-14 activity. In contrast to decorin, the glycosylated form of lumican was able to significantly decrease MMP-14 activity in B16F1 melanoma cells. Our results suggest that a direct interaction occurs between lumican and MMP-14. Lumican behaves as a competitive inhibitor which leads to a complete blocking of the activity of MMP-14. It binds to the catalytic domain of MMP-14 with moderate affinity (KD∼275 nM). Lumican may protect collagen against MMP-14 proteolysis, thus influencing cell-matrix interaction in tumor progression.
MATERIALS
Product Number
Brand
Product Description
Supelco
Calcium standard for AAS, analytical standard, 1.000 g/L Ca+2 in hydrochloric acid, traceable to BAM
Sigma-Aldrich
Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%