Skip to Content
Merck
  • Anthocyanins and antioxidant capacities of six Chilean berries by HPLC-HR-ESI-ToF-MS.

Anthocyanins and antioxidant capacities of six Chilean berries by HPLC-HR-ESI-ToF-MS.

Food chemistry (2015-01-28)
Javier E Ramirez, Ricardo Zambrano, Beatriz Sepúlveda, Edward J Kennelly, Mario J Simirgiotis
ABSTRACT

The HPLC profiles of six fruits endemic of the VIII region of Chile were investigated using high resolution mass analysis (HR-ToF-ESI-MS). The anthocyanin fingerprints generated for the fruits were compared and the antioxidant capacities measured by the scavenging of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and correlated with the inhibition of lipid peroxidation in human erythrocytes (LP) and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Several anthocyanins were identified, including 3-O-glycosides derivatives of delphinidin, cyanidin, petunidin, peonidin and malvidin. Three phenolic acids (feruloyl-quinic acid, chlorogenic acid, and neochlorogenic acid) and five flavonols (hyperoside, isoquercitrin, quercetin, rutin, myricetin and isorhamnetin) were also identified. Calafate fruits showed the highest antioxidant activity. However, the highest LP activity was found for Chilean blueberries (>95%) followed by calafate fruits (91.27%) and luma (83.4%).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetonitrile, ReagentPlus®, 99%
Sigma-Aldrich
Sodium nitrite, ReagentPlus®, ≥99.0%
USP
Phenol, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Ultrapure Acetonitrile
Sigma-Aldrich
Sodium nitrite, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥97%
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%
Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
Acetonitrile, suitable for HPLC-GC, ≥99.8% (GC)
USP
Residual Solvent Class 2 - Acetonitrile, United States Pharmacopeia (USP) Reference Standard
Supelco
Sodium carbonate concentrate, Na2CO3 72 mM in water, IC eluent concentrate (20x) for Metrosep A Supp 7
Supelco
Sodium nitrite, analytical standard
Sigma-Aldrich
Formic acid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
Sigma-Aldrich
Formic acid, puriss., meets analytical specifications of DAC, FCC, 98.0-100%
Sigma-Aldrich
Acetonitrile, suitable for DNA synthesis, ≥99.9% (GC)
Sigma-Aldrich
Sodium nitrite, ACS reagent, ≥97.0%
Sigma-Aldrich
Formic acid, ACS reagent, ≥88%
Sigma-Aldrich
Acetonitrile, ≥99.5% (GC)
Sigma-Aldrich
Acetonitrile, biotech. grade, ≥99.93%
Supelco
Sodium carbonate concentrate, 0.1 M Na2CO3 in water, eluent concentrate for IC
Supelco
Phenol solution, 5000 μg/mL in methanol, certified reference material
Sigma-Aldrich
Trichloroacetic acid solution, 6.1 N
Supelco
Gallic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Sodium hydroxide-16O solution, 20 wt. % in H216O, 99.9 atom % 16O
Supelco
Nitrite ion standard solution, 0.1 M NO2-, for ion-selective electrodes
Sigma-Aldrich
Formic acid solution, BioUltra, 1.0 M in H2O
Sigma-Aldrich
Xanthine, BioUltra, ≥99%
Sigma-Aldrich
Liquified Phenol, ≥89.0%
Sigma-Aldrich
Xanthine, ≥99.5% (HPLC), purified by recrystallization
Sigma-Aldrich
DL-Leucine, ≥99% (HPLC)
Sigma-Aldrich
Phenol, for molecular biology