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  • Conformational changes of the histidine ATP-binding cassette transporter studied by double electron-electron resonance spectroscopy.

Conformational changes of the histidine ATP-binding cassette transporter studied by double electron-electron resonance spectroscopy.

Biochimica et biophysica acta (2014-03-04)
Michael Sippach, Daniela Weidlich, Daniel Klose, Christoph Abé, Johann Klare, Erwin Schneider, Heinz-Jürgen Steinhoff
ABSTRACT

The conformational dynamics of the histidine ABC transporter HisQMP2 from Salmonella enterica serovar Typhimurium, reconstituted into liposomes, is studied by site-directed spin labeling and double electron-electron resonance spectroscopy in the absence of nucleotides, in the ATP-bound, and in the post-hydrolysis state. The results show that the inter-dimer distances as measured between the Q-loops of HisP2 in the intact transporter resemble those determined for the maltose transporter in all three states of the hydrolysis cycle. Only in the presence of liganded HisJ the closed conformation of the nucleotide binding sites is achieved revealing the transmembrane communication of the presence of substrate. Two conformational states can be distinguished for the periplasmic moiety of HisQMP2 as detected by differences in distributions of interspin distances between positions 86 and 96 or 104 and 197. The observed conformational changes are correlated to proposed open, semi-open and closed conformations of the nucleotide binding domains HisP2. Our results are in line with a rearrangement of transmembrane helices 4 and 4' of HisQM during the closed to the semi-open transition of HisP2 driven by the reorientation of the coupled helices 3a and 3b to occur upon hydrolysis.

MATERIALS
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Product Description

Supelco
L-Histidine, Pharmaceutical Secondary Standard; Certified Reference Material
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EN300-7431116-100MG100 mg
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EN300-7431116-250MG250 mg
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EN300-7431116-500MG500 mg
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PLN 3,425.85
EN300-7431116-1G1 g
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PLN 4,338.45
EN300-7431116-2.5G2.5 g
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EN300-7431116-5G5 g
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PLN 12,189.15
EN300-7431116-10G10 g
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PLN 17,980.65
Supelco
L-Histidine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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EN300-7431116-100MG100 mg
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PLN 1,635.75
EN300-7431116-250MG250 mg
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PLN 2,250.00
EN300-7431116-500MG500 mg
Ships in 14 days - from Aldrich Partner.
PLN 3,425.85
EN300-7431116-1G1 g
Ships in 14 days - from Aldrich Partner.
PLN 4,338.45
EN300-7431116-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
PLN 8,304.75
EN300-7431116-5G5 g
Ships in 14 days - from Aldrich Partner.
PLN 12,189.15
EN300-7431116-10G10 g
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PLN 17,980.65
Sigma-Aldrich
L-Histidine, suitable for cell culture, meets EP, USP testing specifications, from non-animal source
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EN300-7431116-100MG100 mg
Ships in 14 days - from Aldrich Partner.
PLN 1,635.75
EN300-7431116-250MG250 mg
Ships in 14 days - from Aldrich Partner.
PLN 2,250.00
EN300-7431116-500MG500 mg
Ships in 14 days - from Aldrich Partner.
PLN 3,425.85
EN300-7431116-1G1 g
Ships in 14 days - from Aldrich Partner.
PLN 4,338.45
EN300-7431116-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
PLN 8,304.75
EN300-7431116-5G5 g
Ships in 14 days - from Aldrich Partner.
PLN 12,189.15
EN300-7431116-10G10 g
Ships in 14 days - from Aldrich Partner.
PLN 17,980.65
SAFC
L-Histidine
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SKUVendor SKUPack SizeAvailabilityPriceQuantity
EN300-7431116-100MG100 mg
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PLN 1,635.75
EN300-7431116-250MG250 mg
Ships in 14 days - from Aldrich Partner.
PLN 2,250.00
EN300-7431116-500MG500 mg
Ships in 14 days - from Aldrich Partner.
PLN 3,425.85
EN300-7431116-1G1 g
Ships in 14 days - from Aldrich Partner.
PLN 4,338.45
EN300-7431116-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
PLN 8,304.75
EN300-7431116-5G5 g
Ships in 14 days - from Aldrich Partner.
PLN 12,189.15
EN300-7431116-10G10 g
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PLN 17,980.65
Sigma-Aldrich
L-Histidine, ReagentPlus®, ≥99% (TLC)
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EN300-7431116-100MG100 mg
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PLN 1,635.75
EN300-7431116-250MG250 mg
Ships in 14 days - from Aldrich Partner.
PLN 2,250.00
EN300-7431116-500MG500 mg
Ships in 14 days - from Aldrich Partner.
PLN 3,425.85
EN300-7431116-1G1 g
Ships in 14 days - from Aldrich Partner.
PLN 4,338.45
EN300-7431116-2.5G2.5 g
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PLN 8,304.75
EN300-7431116-5G5 g
Ships in 14 days - from Aldrich Partner.
PLN 12,189.15
EN300-7431116-10G10 g
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PLN 17,980.65
Sigma-Aldrich
L-Histidine, BioUltra, ≥99.5% (NT)
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EN300-7431116-100MG100 mg
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PLN 1,635.75
EN300-7431116-250MG250 mg
Ships in 14 days - from Aldrich Partner.
PLN 2,250.00
EN300-7431116-500MG500 mg
Ships in 14 days - from Aldrich Partner.
PLN 3,425.85
EN300-7431116-1G1 g
Ships in 14 days - from Aldrich Partner.
PLN 4,338.45
EN300-7431116-2.5G2.5 g
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PLN 8,304.75
EN300-7431116-5G5 g
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PLN 12,189.15
EN300-7431116-10G10 g
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PLN 17,980.65
Histidine, European Pharmacopoeia (EP) Reference Standard
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EN300-7431116-100MG100 mg
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PLN 1,635.75
EN300-7431116-250MG250 mg
Ships in 14 days - from Aldrich Partner.
PLN 2,250.00
EN300-7431116-500MG500 mg
Ships in 14 days - from Aldrich Partner.
PLN 3,425.85
EN300-7431116-1G1 g
Ships in 14 days - from Aldrich Partner.
PLN 4,338.45
EN300-7431116-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
PLN 8,304.75
EN300-7431116-5G5 g
Ships in 14 days - from Aldrich Partner.
PLN 12,189.15
EN300-7431116-10G10 g
Ships in 14 days - from Aldrich Partner.
PLN 17,980.65