Quantification and stability of salbutamol in human urine.

Quantification and stability of salbutamol in human urine.

Journal of separation science (2004-09-01)

Guro Forsdahl, Günter Gmeiner

ABSTRACT

A sensitive method for the quantification of free salbutamol in human urine is described. Sample clean up is performed using SPE on a mixed phase extraction column. Derivatisation is performed with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) and the extract is analysed by GC-MS. The method was found to be suitable for use in the doping field, where a cut-off limit of 1 microg salbutamol/mL urine is set by the International Olympic Committee (IOC) and approved by the World Anti-Doping Agency (WADA). Above that value a doping violation occurs. In addition, the stability of salbutamol in human urine has been evaluated.

MATERIALS

Product Number

Brand

Product Description

M-132

Supelco

MSTFA Reagent, ampule of 10 × 1.2 mL, analytical standard, Cerilliant^®

394866

Supelco

N-Methyl-N-(trimethylsilyl)trifluoroacetamide, synthesis grade

69479

Supelco

N-Methyl-N-(trimethylsilyl)trifluoroacetamide, for GC derivatization, LiChropur^™, ≥98.5%

69478

Supelco

N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane, for GC derivatization, LiChropur^™

M7891

Supelco

N-Methyl-N-(trimethylsilyl)trifluoroacetamide, BioReagent, for silylations, LiChropur^™