- LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus.
LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus.
Antimicrobial agents and chemotherapy (2010-09-22)
Jody L Floyd, Kenneth P Smith, Sanath H Kumar, Jared T Floyd, Manuel F Varela
PMID20855745
ABSTRACT
A multidrug efflux pump designated LmrS (lincomycin resistance protein of Staphylococcus aureus), belonging to the major facilitator superfamily (MFS) of transporters, was cloned, and the role of LmrS in antimicrobial efflux was evaluated. The highest relative increase in MIC, 16-fold, was observed for linezolid and tetraphenylphosphonium chloride (TPCL), followed by an 8-fold increase for sodium dodecyl sulfate (SDS), trimethoprim, and chloramphenicol. LmrS has 14 predicted membrane-spanning domains and is homologous to putative lincomycin resistance proteins of Bacillus spp., Lactobacillus spp., and Listeria spp.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate
Sigma-Aldrich
Rifampicin, suitable for plant cell culture, BioReagent, ≥95% (HPLC), powder or crystals
Sigma-Aldrich
Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)