CMC0006

SIG10 ULTRA Electrocompetent Cells

for BAC & cosmid cloning

• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• biological source: Escherichia coli
• grade: for molecular biology
• form: buffered aqueous solution
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: electrocompetent; transformation efficiency: ≥4 x 10¹⁰ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The SIG10 ULTRA Electrocompetent Cells have the highest transformation efficiency available. They are to be used for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

• share the most useful genetic elements of standard cloning strains like DH5α^™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
• provide the performance researchers need with ease of use.
• provide solutions for a wide range of applications at economical prices.
• Blue - white screening

Components

• SIG10 ULTRA electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

Legal Information

DH5α is a trademark of Invitrogen Corp.

Safety Information

• Storage Class Code: 10 - Combustible liquids