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C1562

Sigma-Aldrich

Freezing container, Nalgene® Mr. Frosty

H × diam. 86 mm × 117 mm

Synonym(s):

freezer container, freezer storage, storage container

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About This Item

UNSPSC Code:
41122800
NACRES:
NB.22
Pricing and availability is not currently available.

feature

to hold 18 x 1.0 to 2.0 mL tubes

packaging

pack of 1 ea

manufacturer/tradename

Nalgene 5100-0001

H × diam.

86 mm × 117 mm

General description

Polycarbonate container, blue high-density polyethylene closure, white high-density polyethylene vial holder, and foam insert.

Provides the critical, repeatable, 1°C/min cooling rate required for successful cryopreservation of cells. Easy to use in any mechanical freezer. Container is imprinted with graphic instructions. Requires only isopropyl alcohol. Rigid vial holder keeps vials from contacting alcohol, preventing contamination by wicking. Vial holder floats to allow thawing in a water bath. Numbers molded for identification.

All components withstand repeated freeze/thaw cycles. Stackable.

Application

Freezing container, Nalgene™ Mr. Frosty may be used for freezing plant cells[1], protoplasts [2], PBMC (peripheral blood mononuclear cell) [3].

Compatibility

Holds 18 x 1.2 and 2.0 mL cryovials

Legal Information

Nalgene is a registered trademark of Thermo Fisher Scientific or its subsidiaries

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Certificates of Analysis (COA)

Lot/Batch Number

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Vanessa Cristina de Oliveira et al.
PloS one, 15(7), e0235856-e0235856 (2020-07-11)
Gene editing in large animal models for future applications in translational medicine and food production must be deeply investigated for an increase of knowledge. The mitochondrial transcription factor A (TFAM) is a member of the HMGB subfamily that binds to
Helen A Fletcher et al.
Vaccine, 26(41), 5269-5275 (2008-08-07)
In clinical trials recombinant-modified vaccinia virus Ankara expressing the Mycobacterium tuberculosis antigen 85A (MVA85A) induces approximately 10 times more effector T cells than any other recombinant MVA vaccine. We have found that in BCG primed subjects MVA85A vaccination reduces transforming
Arend F van Peer et al.
Applied and environmental microbiology, 75(5), 1243-1247 (2008-12-31)
Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, is widely used as a selection marker for transformation. Schizophyllum commune grows on 25 microg of phleomycin ml(-1) after
Ji-Suk Cho et al.
Applied microbiology and biotechnology, 73(6), 1470-1476 (2006-10-06)
Transgenic suspension cells of Oryza sativa L. cv. Dongjin utilized as a host for producing recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) were preserved in liquid nitrogen (-196 degrees C) after slow prefreezing in a deep freezer (-70 degrees C).
Lise M van Wijk et al.
Cancers, 12(10) (2020-10-03)
Recent studies have shown that the efficacy of PARP inhibitors in epithelial ovarian carcinoma (EOC) is related to tumor-specific defects in homologous recombination (HR) and extends beyond BRCA1/2 deficient EOC. A robust method with which to identify HR-deficient (HRD) carcinomas
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Questions

1–10 of 10 Questions  

  1. What frequency of replacing isopropanol in the Mr. Frosty container?

    1 answer

    1. It is recommended that the Isopropanol (100%) be replaced after every fifth use. The Mr. Frosty system depends on the specific heat capacity of alcohol in order to provide the optimum cooling rate of 1°C per minute. After several cooling and warming cycles, enough vapor condensation has taken place that much of the alcohol in the reservoir has been replaced by water. This process affects the heat capacity of the assembled unit such that the cooling rate may be affected.

      Helpful?

  2. I've seen the video for the proper usage of the Mr Frosty but I still have a question, is it possible to use it if it's already at -80°C? Does it cold down the vials gradually if the container is already frozen? Thanks.

    1 answer

    1. No, this product should be thawed completely and allowed to come to room temperature before attempting to freeze any cells. The vials will not cool slowly if the container is already cold.

      Helpful?

  3. Is the foam insert that comes with product C1562 intended to be used or discarded?

    1 answer

    1. As per the supplier instructions, the foam insert is to be discarded as it solely serves as shipping protection.

      Helpful?

  4. What is the function of the foam insert? Do I use it during the freezing process or should it be discarded and never be used? Why is it there from the start??

    1 answer

    1. The foam insert can be discarded. Please see the link below to review a brief video on proper usage:
      https://www.youtube.com/watch?v=44mlLL3D4XQ

      Helpful?

  5. Hi, How can I speed up thawing of the Mr Frosty (because sometimes we need to re-use it the same day)? Is it okay to thaw it in a warm water bath, and then let it cool for a bit to room temperature?

    1 answer

    1. According to the manufacturer, thawing in a warm water bath should not be a problem for this product. However, there is a potential for cracking if the temperature difference is too great due to thermal stresses. It is recommended to thoroughly dry the Mr. Frosty before refreezing.

      Helpful?

  6. Can I use Product C1562, Freezing container, Nalgene®, with 5 mL freezing vials?

    1 answer

    1. Product No. C1562 is designed for use with 1.2 and 2 mL freezing vials.  It cannot be used with 5 mL freezing vials. Nalgene no longer offers the Mr. Frosty in the 5 mLsize.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the rate of cooling of the cell suspension in Product C1562, Freezing container, Nalgene®?

    1 answer

    1. The rate of cooling of 1°C/minute is based on the use of isopropanol as the alcohol bath.

      Helpful?

  9. How do I freeze my cells in a Nalgene® Mr. Frosty, freezing container, product C1562?

    1 answer

    1. Listed below are instructions for the Nalgene® Mr. Frosty freezing container.Fill the container with room-temperature 100% isopropyl alcohol to the indicated fill line and discard the foam insert and tube holder. Insert your sample tubes into the tube holder and place the Mr. Frosty container in -70°C freezer for a minimum of 4 hours. Remove frozen tubes and place in long term storage freezer, at or below -130°C.Note: When not in use, the Mr. Frosty container should be stored at room temperature.

      Helpful?

  10. What alcohol should I use with Mr. Frosty, Product C1562 (Freezing container, Nalgene®)?

    1 answer

    1. We recommend using 2-propanol, commonly known as isopropanol. Among our product listings for 2-propanol we offer Product No. 190764.

      Helpful?

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