03-179
RIPAb+ SUZ12 - RIP Validated Antibody and Primer Set
from mouse
Synonym(s):
ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, joined to JAZF1, suppressor of zeste 12 homolog (Drosophila)
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.52
biological source
mouse
Quality Level
clone
monoclonal
species reactivity
human
manufacturer/tradename
RIPAb+
Upstate®
technique(s)
RIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... SUZ12(23512)
General description
SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates ′Lys-9′ and ′Lys-27′ residues of histone H3.
Specificity
SUZ12
Immunogen
Full length, recombinant human SUZ12.
Application
Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 5 µg of a normal mouse IgG (Cat. #CS200621), or 5 µg of Anti-SUZ12 antibody (Cat. # CS203201).
20 percent of the precipitated proteins (lane 1: mouse IgG, lane 2: SUZ12) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SUZ12 antibody (Cat. # CS203201, 1:1000). Proteins were visualized using One-Step IP-Western kit (GenScript Cat. # L00232)
Arrow indicates SUZ12 (Figure 2).
Western Blot Analysis:
Representative lot data.
Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with anti-SUZ12 (1:1,000 dilution).
Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection.
Arrow SUZ12 (~95 kDa) (Figure 3).
Representative lot data.
RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 5 µg of a normal mouse IgG (Cat. #CS200621), or 5 µg of Anti-SUZ12 antibody (Cat. # CS203201).
20 percent of the precipitated proteins (lane 1: mouse IgG, lane 2: SUZ12) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SUZ12 antibody (Cat. # CS203201, 1:1000). Proteins were visualized using One-Step IP-Western kit (GenScript Cat. # L00232)
Arrow indicates SUZ12 (Figure 2).
Western Blot Analysis:
Representative lot data.
Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with anti-SUZ12 (1:1,000 dilution).
Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection.
Arrow SUZ12 (~95 kDa) (Figure 3).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
This RIPAb+ SUZ12 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Packaging
10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).
Quality
RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG , or 5 µg of Anti-SUZ12 antibody and the Magna RIP® RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of SUZ12-associated RNA was verified by qPCR using RIP Primers lincSFPQ, (Figure 1)
Please refer to the Magna RIP (Cat. # 17-700) or EZ-Magna RIP (Cat. # 17-701) protocol for experimental details.
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG , or 5 µg of Anti-SUZ12 antibody and the Magna RIP® RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of SUZ12-associated RNA was verified by qPCR using RIP Primers lincSFPQ, (Figure 1)
Please refer to the Magna RIP (Cat. # 17-700) or EZ-Magna RIP (Cat. # 17-701) protocol for experimental details.
Target description
95 kDa
Physical form
Anti-SUZ12 (Mouse Monoclonal) -
One vial containing 50 μg of protein A purified antibody in PBS containing 0.1% azide, before the addition of 30% glycerol. Store at -20°C.
Normal Mouse IgG -
One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.
RIP Primers, lincSFPQ -
One vial containing 75 μL of 5 μM of each primer specific for the human lincRNA SFPQ. Store at -20°C.
FOR: TCG TAC TGT TAG GCC CTT GG
REV: AAC CTT GCA TGA AGA GCA CC
One vial containing 50 μg of protein A purified antibody in PBS containing 0.1% azide, before the addition of 30% glycerol. Store at -20°C.
Normal Mouse IgG -
One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.
RIP Primers, lincSFPQ -
One vial containing 75 μL of 5 μM of each primer specific for the human lincRNA SFPQ. Store at -20°C.
FOR: TCG TAC TGT TAG GCC CTT GG
REV: AAC CTT GCA TGA AGA GCA CC
Format: Purified
Protein A purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human lincRNA SFPQ.
Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human lincRNA SFPQ.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MAGNA RIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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