The stability of this product in solution has not been evaluated. However, 172426 Glucoamylase (syn. Amyloglucosides) from Rhizopus, notes that solutions are stable when stored frozen in aliquots at -20°C for up to 3 months. Similar stability would be expected for A7420. Avoid freeze/thaw cycles of solutions.
A7420
Amyloglucosidase from Aspergillus niger
lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose
Synonym(s):
1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase
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About This Item
Pricing and availability is not currently available.
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form
lyophilized powder
Quality Level
specific activity
30-60 units/mg protein (biuret)
composition
protein, ≥80%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
Greener Alternative Product
impurities
≤0.02% glucose
greener alternative category
, Enabling
storage temp.
−20°C
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle [1].
Biochem/physiol Actions
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides.[2] Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.[3][4]
Unit Definition
One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.
Physical form
Lyophilized powder containing less than 0.02% glucose
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
inhibitor
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
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What is the shelf life of a solution (1 mg/mL in water) of Product A7420, Amyloglucosidase from Aspergillus niger and how should it be stored?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can a solution of Product A7420, Amyloglucosidase from Aspergillus niger, be prepared?
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A 1 mg/mL solution may be prepared in water.
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What is the molecular weight of Product A7420, Amyloglucosidase from Aspergillus niger?
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Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).
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Does Product A7420, Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?
1 answer-
The starting material for Product No. A7420 is Product No. A3042, which is a preparation of the enzyme in a 1 M glucose solution. This is dialyzed against water until the glucose concentration is less than 0.02%, and then lyophilized.
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