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SAB4200602

Sigma-Aldrich

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

~1.0 mg/mL, clone 5C5, purified from hybridoma cell culture

Synonym(s):

Anti-AA959943, Anti-ACTA1, Anti-ALPHA SKELETAL ACTIN, Anti-ALPHA1 ACTIN, Anti-ASMA, Anti-Acta-2, Anti-Acts, Anti-Actsk-1, Anti-CFTD, Anti-CFTDM, Anti-Fetal isoform of skeletal alpha-actin, G-actin, Anti-MPFD, Anti-NEM1, Anti-RP5-1068B5.2, Anti-actin, alpha 1, skeletal muscle

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

5C5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

human, carp, bovine, frog, sheep, rabbit, rat, snake, guinea pig

concentration

~1.0 mg/mL

technique(s)

immunoblotting: suitable
immunohistochemistry: 1-2 μg/mL using formalin-fixed, paraffin embedded human tongue
indirect ELISA: suitable

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTA1(58)

General description

Monoclonal Anti-Actin (α-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rabbit striated muscle. Actin is one of the major cytoskeletal proteins in eukaryotic cells.

Immunogen

purified rabbit striated muscle

Application

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has been used in:
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Biochem/physiol Actions

Actin plays an essential role in various cellular processes including cell migration, cytokinesis, vesicle transport, and contractile force generation. Pathological conditions influenced by genetic and/or environmental factors causes variations in the sarcomeric actin filaments. These variations in the actin filaments leads to the development of congenital myopathy, a genetic muscle disorder.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Beclin 1 knockdown retards re-endothelialization and exacerbates neointimal formation via a crosstalk between autophagy and apoptosis
Ye LX, et al.
Atherosclerosis, 237(1), 146-154 (2014)
The actin cytoskeleton and actin-based motility
Svitkina T
Cold Spring Harbor Perspectives in Biology, 10(1), a018267-a018267 (2018)
O Skalli et al.
The American journal of pathology, 130(3), 515-531 (1988-03-01)
A series of 15 rhabdomyosarcomas was examined by light microscopy, transmission electron microscopy, two-dimensional gel electrophoresis (2D-GE) and indirect immunofluorescence, the latter using monoclonal or affinity-purified polyclonal antibodies to desmin, vimentin, alpha-smooth muscle and alpha-sarcomeric (alpha-sr) actins. By light microscopy
Dynamic regulation of sarcomeric actin filaments in striated muscle
Ono S
Cytoskeleton (Hoboken, N.J.), 67(11) (2010)
Actin, a central player in cell shape and movement
Pollard TD and Cooper JA
Science, 326(5957), 1208-1212 (2009)

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