Skip to Content
Merck
All Photos(1)

Documents

P4860

Sigma-Aldrich

Protease from Bacillus licheniformis

≥2.4 U/g

Synonym(s):

Alcalase® 2.4L, Proteinase from Bacillus licheniformis, Subtilisin A

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9014-01-1
Enzyme Commission number:
3.4.21.62 (BRENDA, IUBMB)
EC Number:
232-752-2
MDL number:
MFCD00132092
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

form

aqueous solution

specific activity

≥2.4 U/g

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations.
The enzyme from Sigma has been used to prepare chicken (leg) bone protein hydrolysates while evaluating ACE inhibitory peptides. It has also been used for the hydrolysis of Chlorella pyrenoidosa protein extracts during a study of their antitumor activities.

Biochem/physiol Actions

Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Analysis Note

Activity expressed in Anson Units

Legal Information

A product of Novozyme Corp.
Alcalase is a registered trademark of Novozymes Corp.

Signal Word

Danger

Hazard Statements

H315,H318,H334,H335,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service