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L8777

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder, BioReagent, suitable for cell culture

Synonym(s):

PWM, Pokeweed mitogen

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About This Item

MDL number:
MFCD00161973
UNSPSC Code:
12352202
NACRES:
NA.32

sterility

aseptically filled

Quality Level

200

product line

BioReagent

form

lyophilized powder

potency

≤0.3 μg/mL mitogenic activity

technique(s)

cell culture | mammalian: suitable

solubility

PBS: 1 mg/mL

storage temp.

2-8°C

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General description

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Application

Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).

Biochem/physiol Actions

Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Quality

Partially purified TCA precipitate.
Essentially salt-free.

Analysis Note

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Precautionary Statements

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Caroline Leeth et al.
Animals : an open access journal from MDPI, 11(10) (2021-10-24)
Immunological studies in the horse are frequently hampered by lack of environmental control, complicated study design, and ethical concerns when performing high risk studies. The purpose of the current study was to investigate the utility of a xenograft model for
Tomomi Fujii et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 4), 665-673 (2004-03-25)
Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
Samira Rezaei et al.
Scientific reports, 10(1), 7471-7471 (2020-05-06)
In the routine commercial karyotype analysis on 5,481 boars, we identified 32 carriers of mosaic reciprocal translocations, half of which were carrying a specific recurrent translocation, mos t(7;9). An additional 7 mosaic translocations were identified through lymphocyte karyotype analysis from
Sharon N Cox et al.
Kidney international, 78(4), 396-407 (2010-05-21)
Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis worldwide. The basic defect lies within the IgA immune system and in peripheral blood leukocytes, rather than local kidney abnormalities. To define the intracellular mechanisms leading to the
A randomized, placebo-controlled phase I study assessing the safety and immunogenicity of a Pseudomonas aeruginosa hybrid outer membrane protein OprF/I vaccine (IC43) in healthy volunteers.
Westritschnig K, Hochreiter R, Wallner G, et al.
Human Vaccines & Immunotherapeutics, 10(1), 170-183 (2014)
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