L0159

Anti-Luciferase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti Luciferase Antibody, Firefly Luciferase Antibody, Luciferase Antibody, Luciferase Antibody - Anti-Luciferase antibody produced in rabbit
• MDL number: MFCD01092407
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• packaging: antibody small pack of 25 μL
• technique(s): indirect immunofluorescence: 1:500-1:1,000 using eukaryotic cells transfected with a plasmid bearing the luciferase gene; western blot: 1:10,000 using purified Luciferase
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Analysis of gene expression is commonly assayed by transient transfection. These systems are usually based on the use of fusion genes which are inserted into cells, and expression of the gene is assayed within 48 hours after introduction of DNA. Usually the fusion consists of the promoter binding site or enhancer sequence under study which is attached to a reporter gene. The amount of the reporter protein synthesized under the experimental conditions is presumed to reflect the ability of the sequences studied to direct or promote transcription.
Luciferase has become one of the more widely used reporter enzymes. The reporter plasmid contains the gene from the firefly Photinus pyralis. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg⁺² and ATP. Mixing these reagents with the cell extract containing luciferase results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The luciferase assay is fast and sensitive and does not require a radioactive substrate as in the CAT assay. A disadvantage of the luciferase assay is that it requires a rather expensive instrument, the luminometer, to measure enzyme activity.

Specificity

The antibody identifies recombinant luciferase in eukaryotic cells transfected with a plasmid bearing the luciferase gene.

Immunogen

firefly (Photinus pyralis) luciferase.

Application

Anti-Luciferase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable