Skip to Content
Merck
All Photos(2)

Documents

H7630

Sigma-Aldrich

Hyaluronic acid sodium salt from bovine vitreous humor

Synonym(s):

Poly(β-glucuronic acid-[1→3]-β-N-acetylglucosamine-[1→4]), alternating

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Linear Formula:
(C14H21NaNO11)n
CAS Number:
9067-32-7
MDL number:
MFCD01773053
UNSPSC Code:
12352201
NACRES:
NA.77

biological source

bovine (vitreous humor)

form

powder

color

white

solubility

water: ~5 g/L

storage temp.

−20°C

InChI

1S/C28H44N2O23.Na/c1-5(33)29-9-18(11(35)7(3-31)47-25(9)46)49-28-17(41)15(39)20(22(53-28)24(44)45)51-26-10(30-6(2)34)19(12(36)8(4-32)48-26)50-27-16(40)13(37)14(38)21(52-27)23(42)43;/h7-22,25-28,31-32,35-41,46H,3-4H2,1-2H3,(H,29,33)(H,30,34)(H,42,43)(H,44,45);/q;+1/t7-,8-,9-,10-,11-,12-,13+,14+,15-,16-,17-,18-,19-,20+,21+,22+,25-,26+,27-,28-;/m1./s1

InChI key

YWIVKILSMZOHHF-QJZPQSOGSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Hyaluronic acid sodium salt from bovine vitreous humor has been used:
  • as a component for the generation of artificial synovial fluid
  • to investigate its role in inducing oxidative stress and interleukin 8(IL-8) production in human nasal RPMI 2650 cells
  • to test its effect on B cell proliferation
  • as a substrate in neuraminidase assay of morbilliviruses virus

Biochem/physiol Actions

High molecular mass polymer composed of repeating dimeric units of glucuronic acid and N-acetyl glucosamine which forms the core of complex proteoglycan aggregates found in extracellular matrix.
Hyaluronic acid (HA) is abundantly present in the bovine vitreous humor. It corresponds to a molecular weight of 69293.9Da and has an isoelectric pH of 5.82. Bovine HA has high levels of endotoxins and it polydisperse with DNA and RNA contaminants. The enzyme HA synthases at the plasma membrane (HASs) aids in the synthesis of circulatory HA. HA is implicated in many physiological processes including inflammation, immune regulation, adhesion, embryo development and tumor progression.

Other Notes

To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yi-Wen Lin et al.
Molecules (Basel, Switzerland), 25(19) (2020-10-01)
Osteoarthritis (OA) is the most common joint disease type and is accompanied by varying degrees of functional limitation. Both hyaluronic acid (HA) joint injections and pain relievers are efficient treatments for early-stage osteoarthritis. However, for the decomposition by hyaluronidase and
Role of the biomolecular interactions in the structure and tribological properties of synovial fluid
Mirea DA, et al.
Tribology International, 59(4), 302-311 (2013)
Sequence and structure alignment of Paramyxoviridae attachment proteins and discovery of enzymatic activity for a morbillivirus hemagglutinin.
Langedijk JP, et al.
Journal of virology, 71(8), 6155-6167 (1997)
Evaluation of hyaluronic acid in cattle: physiological variations related to age, periparturition and in clinical cases of paratuberculosis
Jolly A, et al.
Journal of Veterinary Science and Technology, 7(4), 342-346 (2016)
Evaluation of Hyaluronan from Different Sources: Streptococcus z ooepidemicus, Rooster Comb, Bovine Vitreous, and Human Umbilical Cord
Shiedlin A, et al.
Biomacromolecules, 5(6), 2122-2127 (2004)
View All Related Papers

Articles

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

See All

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service