G4040
Anti-Gα i3 antibody produced in rabbit
whole antiserum, buffered aqueous solution
Synonym(s):
Anti-87U6, Anti-ARCND1, Anti-HG1A
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~40 kDa
species reactivity
mouse, human, rat, hamster
technique(s)
immunoprecipitation (IP): suitable
western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GNAI3(2773)
mouse ... Gnai3(14679)
rat ... Gnai3(25643)
General description
G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.
Specificity
Recognizes Gαi3 and Gα0 G-proteins.
Immunogen
synthetic peptide corresponding to the C-terminal region of Gαi3.
Application
Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.
Biochem/physiol Actions
Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca+2 mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.
Physical form
Solution of whole antiserum diluted in PBS, pH 7.4.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Andrew Forge et al.
PLoS genetics, 13(3), e1006692-e1006692 (2017-03-28)
Behavioural anomalies suggesting an inner ear disorder were observed in a colony of transgenic mice. Affected animals were profoundly deaf. Severe hair bundle defects were identified in all outer and inner hair cells (OHC, IHC) in the cochlea and in
L J Yang et al.
The Journal of biological chemistry, 266(33), 22451-22458 (1991-11-25)
Treatment of rat hepatocytes with epidermal growth factor (EGF) produced an enhanced tyrosine phosphorylation of the EGF receptor and phospholipase C-gamma (PLC-gamma) in conjunction with the mobilization of Ca2+. Approximately 30% of the total PLC-gamma was tyrosine-phosphorylated with a maximum
Lili Zheng et al.
Cytoskeleton (Hoboken, N.J.), 79(6-8), 64-74 (2022-07-19)
Stereocilia are actin-based projections of hair cells that are arranged in a step like array, in rows of increasing height, and that constitute the mechanosensory organelle used for the senses of hearing and balance. In order to function properly, stereocilia
The many faces of G protein signaling.
H E Hamm
The Journal of biological chemistry, 273(2), 669-672 (1998-02-14)
G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.
M Strathmann et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(23), 9113-9117 (1990-12-01)
Heterotrimeric guanine nucleotide-binding proteins (G proteins) are integral to the signal transduction pathways that mediate the cell's response to many hormones, neuromodulators, and a variety of other ligands. While many signaling processes are guanine nucleotide dependent, the precise coupling between
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