FLE250

Firefly Lantern Extract

for ATP determination

• Synonym(s): FLE
• NACRES: NA.81
• UNSPSC Code: 12352202
• Form: solution

Properties

• Quality Level: 200
• form: solution
• packaging: pkg of 250 mg dried lanterns
• technique(s): toxicology assay: suitable
• storage temp.: −20°C

Description

General description

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.

Application

Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.

Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.

Biochem/physiol Actions

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg²⁺ to yield a light-emitter oxyluciferin.

Preparation Note

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO₄^.

Other Notes

Crude luciferin and luciferase

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable