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F4049

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

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General description

Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.

Application

Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.

Learn more product details in ourFLAG® application portal.

Physical form

Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Emmanuelle Despras et al.
Nature communications, 7, 13326-13326 (2016-11-05)
Translesion polymerase eta (polη) was characterized for its ability to replicate ultraviolet-induced DNA lesions that stall replicative polymerases, a process promoted by Rad18-dependent PCNA mono-ubiquitination. Recent findings have shown that polη also acts at intrinsically difficult to replicate sequences. However
Jun-ichi Asaka et al.
Molecular pharmacology, 71(6), 1487-1493 (2007-03-01)
Multidrug and toxin extrusion 1 (MATE1) has been isolated as an H(+)/organic cation antiporter located at the renal brush-border membranes. Previous studies using rat renal brush-border membrane vesicles indicated that cysteine and histidine residues played critical roles in H(+)/organic cation
Xuechen Lv et al.
Protein & cell, 7(5), 325-337 (2016-04-18)
G protein-coupled receptors (GPCRs) are involved in all human physiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect
Pete Stavropoulos et al.
Journal of immunological methods, 362(1-2), 190-194 (2010-08-31)
The African trypanosome (Trypanosoma brucei) is transmitted by the bite of the tsetse vector to the mammalian bloodstream where it exists as a completely extracellular parasite. As a result of this exposure, the parasite elicits a robust immune response that
Ermelinda Porpiglia et al.
PLoS biology, 10(8), e1001383-e1001383 (2012-09-13)
Erythropoietin (Epo)-induced Stat5 phosphorylation (p-Stat5) is essential for both basal erythropoiesis and for its acceleration during hypoxic stress. A key challenge lies in understanding how Stat5 signaling elicits distinct functions during basal and stress erythropoiesis. Here we asked whether these
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