E5529

EZview^™ Red Streptavidin Affinity Gel

• NACRES: NA.56
• UNSPSC Code: 41106500

Properties

• form: suspension
• Quality Segment: 200
• shelf life: 1 yr
• technique(s): biotinylated protein purification: suitable, western blot: suitable
• matrix: 4% agarose
• pH: 7.2
• capacity: ~10 μg(biotin per ml of packed gel)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.

Application

Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview^™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and MIT-HClis present at 30 PPM and 0.003% (w/v).

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable