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QBL human cell line

IHW Number: 9020, 94070713

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About This Item

UNSPSC Code:
41106514

Pricing and availability is not currently available.

biological source

human blood

ethnicity

Dutch

gender

male

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

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1 of 4

This Item
29752-U29745-U29744-U
agency

EPA TO-1,TO-2,TO-3,TO-17

agency

EPA IP-1B, NIOSH 2549

agency

-

agency

EPA TO-14 (Air Toxics), EPA TO-17

technique(s)

active air sampling: suitable

technique(s)

active air sampling: suitable

technique(s)

active air sampling: suitable

technique(s)

active air sampling: suitable

feature

fritted

feature

fritted, preconditioned

feature

preconditioned

feature

preconditioned

product line

Carbotrap®

product line

Carbotrap®

product line

Carbotrap®

product line

Carbotrap®

packaging

pkg of 10 ea

packaging

pkg of 10 ea

packaging

pkg of 10 ea

packaging

pkg of 10 ea

General description

Consanguineous: Yes

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 2601
Cw: 501
B: 1801
HLA DRA: 101
HLA DRB1: 301
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 201
HLA DPA1: 1
HLA DPB1: 202

Serological Profile

HLA A: 26
HLA A ief: 26.1
HLA C: 5
HLA B: 18
HLA B ief: 18.1
Bw4/bw6: 6
HLA Dw: 3
HLA DR: 3
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 2
HLA DP: 2

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

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