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C3143

Sigma-Aldrich

Chromotrope 2R

Powder

Synonym(s):

2-(Phenylazo)chromotropic acid disodium salt, Acid Red 29

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About This Item

Empirical Formula (Hill Notation):
C16H10N2Na2O8S2
CAS Number:
Molecular Weight:
468.37
Colour Index Number:
16570
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Chromotrope 2R, suitable for modified Gomori Trichrome stain

form

powder

composition

Dye content, ≥70%

color

red to brown

solubility

H2O: 1 mg/mL, dark red

εmax

≥450 at 510-513 nm in methanol

suitability

suitable for modified Gomori Trichrome stain

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1cc(cc2cc(c(\N=N\c3ccccc3)c(O)c12)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H12N2O8S2.2Na/c19-12-8-11(27(21,22)23)6-9-7-13(28(24,25)26)15(16(20)14(9)12)18-17-10-4-2-1-3-5-10;;/h1-8,19-20H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b18-17+;;

InChI key

GXEAXHYQKZAJGB-QIKYXUGXSA-L

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Application

Chromotrope 2R has been used in the Gomori trichrome staining in osteochondral tissues. It has also been used for the staining of eosinophils in lung sections.

Biochem/physiol Actions

Chromotrope 2R is used for the staining of microsporidia in body fluids and stool samples. It is used in modified Gomori′s trichrome staining. Chromotrope 2R has high affinity for phospholipids and is used for the staining of mitochondrial and endoplasmic reticulum membrane in histological samples.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A simple, accurate and highly sensitive spectrophotometric method is proposed for the rapid determination of pipazethate hydrochloride, dextromethorphan hydrobromide and drotaverine hydrochloride using chromotrope 2B (C2B) and chromotrope 2R (C2R). The method consists of extracting the formed ion-associates into chloroform

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